Human EAAT3 (SLC1A1) (NM_004170) AAV Particle

SKU RC206281A1V

AAV ORF Particles, serotype AAV-2, SLC1A1 (Myc-DDK-tagged)-Human solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (SLC1A1), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: EAAT3
• Synonyms: DCBXA; EAAC1; EAAT3; SCZD18
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206281 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGGAAACCGGCGAGGAAAGGATGCGAGTGGAAGCGCTTCCTGAAGAATAACTGGGTGTTGCTGTCCA; CCGTGGCCGCGGTGGTGCTAGGCATTACCACAGGAGTCTTGGTTCGAGAACACAGCAACCTCTCAACTCT; AGAGAAATTCTACTTTGCTTTTCCTGGAGAAATTCTAATGCGGATGCTGAAACTCATCATTTTGCCATTA; ATTATATCCAGCATGATTACAGGTGTTGCTGCACTGGATTCCAACGTATCCGGAAAAATTGGTGTGCGCG; CTGTCGTGTATTATTTCTGTACCACTCTCATTGCTGTTATTCTAGGTATTGTGCTGGTGGTGAGCATCAA; GCCTGGTGTCACCCAGAAAGTGGGTGAAATTGCGAGGACAGGCAGCACCCCTGAAGTCAGTACGGTGGAT; GCCATGTTAGATCTCATCAGGAATATGTTCCCTGAGAATCTTGTCCAGGCCTGTTTTCAGCAGTACAAAA; CTAAGCGTGAAGAAGTGAAGCCTCCCAGCGATCCAGAGATGAACATGACAGAAGAGTCCTTCACAGCTGT; CATGACAACTGCAATTTCCAAGAACAAAACAAAGGAATACAAAATTGTTGGCATGTATTCAGATGGCATA; AACGTCCTGGGCTTGATTGTCTTTTGCCTTGTCTTTGGACTTGTCATTGGAAAAATGGGAGAAAAGGGAC; AAATTCTGGTGGATTTCTTCAATGCTTTGAGTGATGCAACCATGAAAATCGTTCAGATCATCATGTGTTA; TATGCCACTAGGTATTTTGTTCCTGATTGCTGGGAAGATCATAGAAGTTGAAGACTGGGAAATATTCCGC; AAGCTGGGCCTTTACATGGCCACAGTCCTGACTGGGCTTGCAATCCACTCCATTGTAATTCTCCCGCTGA; TATATTTCATAGTCGTACGAAAGAACCCTTTCCGATTTGCCATGGGAATGGCCCAGGCTCTCCTGACAGC; TCTCATGATCTCTTCCAGTTCAGCAACACTGCCTGTCACCTTCCGCTGTGCTGAAGAAAATAACCAGGTG; GACAAGAGGATCACTCGATTCGTGTTACCCGTTGGTGCAACAATCAACATGGATGGGACTGCGCTCTATG; AAGCAGTGGCAGCGGTGTTTATTGCACAGTTGAATGACCTGGACTTGGGCATTGGGCAGATCATCACCAT; CAGTATCACGGCCACATCTGCCAGCATCGGAGCTGCTGGCGTGCCCCAGGCTGGCCTGGTGACCATGGTG; ATTGTGCTGAGTGCCGTGGGCCTGCCCGCCGAGGATGTCACCCTGATCATTGCTGTCGACTGGCTCCTGG; ACCGGTTCAGGACCATGGTCAACGTCCTTGGTGATGCTTTTGGGACGGGCATTGTGGAAAAGCTCTCCAA; GAAGGAGCTGGAGCAGATGGATGTTTCATCTGAAGTCAACATTGTGAATCCCTTTGCCTTGGAATCCACA; ATCCTTGACAACGAAGACTCAGACACCAAGAAGTCTTATGTCAATGGAGGCTTTGCAGTAGACAAGTCTG; ACACCATCTCATTCACCCAGACCTCACAGTTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206281 protein sequence; Red=Cloning site Green=Tags(s); ; MGKPARKGCEWKRFLKNNWVLLSTVAAVVLGITTGVLVREHSNLSTLEKFYFAFPGEILMRMLKLIILPL; IISSMITGVAALDSNVSGKIGVRAVVYYFCTTLIAVILGIVLVVSIKPGVTQKVGEIARTGSTPEVSTVD; AMLDLIRNMFPENLVQACFQQYKTKREEVKPPSDPEMNMTEESFTAVMTTAISKNKTKEYKIVGMYSDGI; NVLGLIVFCLVFGLVIGKMGEKGQILVDFFNALSDATMKIVQIIMCYMPLGILFLIAGKIIEVEDWEIFR; KLGLYMATVLTGLAIHSIVILPLIYFIVVRKNPFRFAMGMAQALLTALMISSSSATLPVTFRCAEENNQV; DKRITRFVLPVGATINMDGTALYEAVAAVFIAQLNDLDLGIGQIITISITATSASIGAAGVPQAGLVTMV; IVLSAVGLPAEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALEST; ILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_004170
• ORF Size: 1572 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_004170.4
• RefSeq Size: 3757 bp
• RefSeq ORF: 1575 bp
• Locus ID: 6505
• UniProt ID: P43005
• Cytogenetics: 9p24.2
• MW: 57.1 kDa