Human Nck beta (NCK2) (NM_003581) AAV Particle

SKU RC202557A1V

AAV ORF Particles, serotype AAV-2, NCK2 (Myc-DDK-tagged)-Human NCK adaptor protein 2 (NCK2), transcript variant 1, 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Nck beta
• Synonyms: GRB4; NCKbeta
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202557 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACAGAAGAAGTTATTGTGATAGCCAAGTGGGACTACACCGCCCAGCAGGACCAGGAGCTGGACATCA; AGAAGAACGAGCGGCTGTGGTTGCTGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCAACAG; GACGGGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGGCTCCCTCGTGAAGAAC; CTGAAGGACACACTAGGCCTCGGCAAGACGCGCAGGAAGACCAGCGCGCGGGATGCGTCCCCCACGCCCA; GCACGGACGCCGAGTACCCCGCCAATGGCAGCGGCGCCGACCGCATCTACGACCTCAACATCCCGGCCTT; CGTCAAGTTCGCCTATGTGGCCGAGCGGGAGGATGAGTTGTCCCTGGTGAAGGGGTCGCGCGTCACCGTC; ATGGAGAAGTGCAGCGACGGTTGGTGGCGGGGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACT; ACGTCTTGGAGGAGGTGGACGAGGCGGCTGCGGAGTCCCCAAGCTTCCTGAGCCTGCGCAAGGGCGCCTC; GCTGAGCAATGGCCAGGGCTCCCGCGTGCTGCATGTGGTCCAGACGCTGTACCCCTTCAGCTCAGTCACC; GAGGAGGAGCTCAACTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAACGACCCCGAGT; GGTGGAAATGCAAAAATGCCCGGGGCCAGGTGGGCCTCGTCCCCAAAAACTACGTGGTGGTCCTCAGTGA; CGGGCCTGCCCTGCACCCTGCGCACGCCCCACAGATAAGCTACACCGGGCCCTCGTCCAGCGGGCGCTTC; GCGGGCAGAGAGTGGTACTACGGGAACGTGACGCGGCACCAGGCCGAGTGCGCCCTCAACGAGCGGGGCG; TGGAGGGCGACTTCCTCATTAGGGACAGCGAGTCCTCGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTC; AGGGAAGAACAAACACTTCAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGCTTCCAC; ACCATGGACGAGCTGGTGGAACACTACAAAAAGGCGCCCATCTTCACCAGCGAGCACGGGGAGAAGCTCT; ACCTCGTCAGGGCCCTGCAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202557 protein sequence; Red=Cloning site Green=Tags(s); ; MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKN; LKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTV; MEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVT; EEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRF; AGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFH; TMDELVEHYKKAPIFTSEHGEKLYLVRALQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_003581
• ORF Size: 1140 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_003581.2
• RefSeq Size: 2517 bp
• RefSeq ORF: 1143 bp
• Locus ID: 8440
• UniProt ID: O43639
• Cytogenetics: 2q12.2
• MW: 42.9 kDa