Human PUS3 (NM_031307) AAV Particle

SKU RC201648A1V

AAV ORF Particles, serotype AAV-2, PUS3 (Myc-DDK-tagged)-Human pseudouridylate synthase 3 (PUS3), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: PUS3
• Synonyms: 2610020J05Rik; FKSG32; MRT55; NEDMIGS
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201648 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGATAATGACACAGACAGAAACCAGACTGAGAAGCTCCTAAAAAGAGTACGAGAACTGGAGCAAG; AGGTGCAAAGACTTAAAAAGGAACAGGCCAAAAATAAGGAGGACTCAAACATTAGAGAAAATTCATCAGG; AGCTGGAAAAACTAAGCGTGCATTTGATTTCAGTGCTCATGGCCGAAGACACGTAGCCCTAAGAATAGCC; TATATGGGCTGGGGATACCAGGGCTTTGCTAGTCAGGAAAACACAAATAATACCATTGAAGAGAAACTGT; TTGAAGCTCTAACCAAGACTCGACTAGTAGAAAGCAGACAGACATCCAACTATCACCGATGTGGGAGAAC; AGATAAAGGAGTTAGTGCCTTTGGACAGGTGATCTCACTTGACCTTCGCTCTCAGTTTCCAAGGGGCAGG; GATTCCGAGGACTTTAATGTAAAAGAGGAGGCTAATGCTGCTGCTGAAGAGATCCGTTATACCCACATTC; TCAATCGGGTACTCCCTCCAGACATCCGTATATTGGCCTGGGCCCCTGTAGAACCAAGCTTCAGTGCTAG; GTTCAGCTGCCTTGAGCGGACTTACCGCTATTTTTTCCCTCGTGCTGATTTAGATATTGTAACCATGGAT; TATGCAGCTCAGAAGTATGTTGGCACCCATGATTTCAGGAACTTGTGTAAAATGGATGTAGCCAACGGTG; TGATTAATTTTCAGAGGACTATTCTATCTGCTCAAGTACAGCTAGTGGGCCAGAGCCCAGGTGAGGGGAG; ATGGCAAGAACCTTTCCAGTTATGTCAGTTTGAAGTGACTGGCCAGGCATTCCTTTATCATCAAGTCCGA; TGTATGATGGCTATCCTCTTTCTGATTGGCCAAGGAATGGAGAAGCCAGAGATTATTGATGAGCTGCTGA; ATATAGAGAAAAATCCCCAAAAGCCTCAATATAGTATGGCTGTAGAATTTCCTCTAGTCTTATATGACTG; TAAGTTTGAAAATGTCAAGTGGATCTATGACCAGGAGGCTCAGGAGTTCAATATTACCCACCTACAACAA; CTGTGGGCTAATCATGCTGTCAAAACTCACATGTTGTATAGTATGCTACAAGGACTGGACACTGTTCCAG; TACCCTGTGGAATAGGACCAAAGATGGATGGAATGACAGAATGGGGAAATGTTAAGCCCTCTGTCATAAA; GCAGACCAGTGCCTTTGTAGAAGGAGTGAAGATGCGCACATATAAGCCCCTCATGGACCGTCCTAAATGC; CAAGGACTGGAATCCCGGATCCAGCATTTTGTACGTAGGGGACGAATTGAGCACCCACATTTATTCCATG; AGGAAGAAACAAAAGCCAAAAGGGACTGTAATGACACACTAGAGGAAGACAATACTAATTTGGAGACACC; AACGAAGAGGGTCTGTGTTGACACAGAAATTAAAAGCATCATT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201648 protein sequence; Red=Cloning site Green=Tags(s); ; MADNDTDRNQTEKLLKRVRELEQEVQRLKKEQAKNKEDSNIRENSSGAGKTKRAFDFSAHGRRHVALRIA; YMGWGYQGFASQENTNNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRSQFPRGR; DSEDFNVKEEANAAAEEIRYTHILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDIVTMD; YAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVR; CMMAILFLIGQGMEKPEIIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKWIYDQEAQEFNITHLQQ; LWANHAVKTHMLYSMLQGLDTVPVPCGIGPKMDGMTEWGNVKPSVIKQTSAFVEGVKMRTYKPLMDRPKC; QGLESRIQHFVRRGRIEHPHLFHEEETKAKRDCNDTLEEDNTNLETPTKRVCVDTEIKSII; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_031307
• ORF Size: 1443 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_031307.2, NP_112597.2
• RefSeq Size: 1862 bp
• RefSeq ORF: 1446 bp
• Locus ID: 83480
• UniProt ID: Q9BZE2
• Cytogenetics: 11q24.2
• MW: 55.6 kDa