Mouse Gpbar1 (NM_174985) AAV Particle

SKU MR227683A1V

AAV ORF Particles, serotype AAV-2, Gpbar1 (Myc-DDK-tagged) - Mouse G protein-coupled bile acid receptor 1 (Gpbar1), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Gpbar1
• Synonyms: BG37; GPCR; GPR131; M-BAR; TGR5
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227683 representing NM_174985; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGACACCCAACAGCACTGAGCTGTCGGCCATTCCCATGGGGGTTCTGGGGCTTTCCTTGGCCCTGG; CAAGCCTCATCGTCATCGCCAACCTGCTCCTGGCCCTAGGCATCGCCCTGGACCGCCACTTGCGCAGCCC; ACCTGCTGGCTGCTTCTTCCTAAGCCTACTACTAGCCGGGCTGCTCACAGGGCTGGCACTGCCCATGCTG; CCTGGGCTATGGAGCCGGAACCATCAGGGCTACTGGTCCTGCCTCCTTCTCCACTTGACCCCCAACTTTT; GTTTCCTTTCCCTGCTTGCCAATCTGCTGCTGGTGCATGGGGAACGCTACATGGCAGTGTTGCAGCCACT; CCGGCCCCATGGAAGTGTGCGGCTAGCCCTGTTCCTCACCTGGGTCAGCTCCCTGTTCTTTGCCAGCCTG; CCTGCTCTGGGCTGGAACCATTGGAGCCCTGATGCCAACTGCAGCTCCCAAGCTGTCTTCCCAGCCCCCT; ACCTCTACCTGGAAGTTTATGGCCTCCTGTTGCCTGCCGTGGGGGCCACTGCCCTTCTCTCTGTCCGCGT; GTTGGCCACTGCCCACCGCCAGCTGTGTGAGATCCGCCGACTGGAGCGGGCAGTGTGCCGCGATGTACCC; TCAACCCTGGCTAGGGCTCTCACCTGGAGGCAGGCTAGGGCACAGGCAGGAGCCACACTGCTCTTCTTGC; TGTGTTGGGGGCCCTATGTGGCCACATTGCTCCTGTCAGTCTTGGCCTATGAGCGTCGCCCACCACTAGG; GCCTGGAACTCTGTTATCGCTCATCTCATTGGGCAGCACCAGTGCTGCCGCTGTGCCTGTGGCCATGGGG; CTGGGTGATCAGCGCTACACAGCCCCCTGGAGGACAGCTGCCCAAAGGTGTCTACGAGTGCTTCGAGGAA; GAGCCAAGAGGGACAATCCAGGCCCCAGCACTGCCTACCACACCAGTAGCCAATGCAGCATTGACCTGGA; CTTGAAT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227683 representing NM_174985; Red=Cloning site Green=Tags(s); ; MMTPNSTELSAIPMGVLGLSLALASLIVIANLLLALGIALDRHLRSPPAGCFFLSLLLAGLLTGLALPML; PGLWSRNHQGYWSCLLLHLTPNFCFLSLLANLLLVHGERYMAVLQPLRPHGSVRLALFLTWVSSLFFASL; PALGWNHWSPDANCSSQAVFPAPYLYLEVYGLLLPAVGATALLSVRVLATAHRQLCEIRRLERAVCRDVP; STLARALTWRQARAQAGATLLFLLCWGPYVATLLLSVLAYERRPPLGPGTLLSLISLGSTSAAAVPVAMG; LGDQRYTAPWRTAAQRCLRVLRGRAKRDNPGPSTAYHTSSQCSIDLDLN; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_174985
• ORF Size: 987 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_174985.1, NP_778150.1
• RefSeq Size: 990 bp
• RefSeq ORF: 990 bp
• Locus ID: 227289
• UniProt ID: Q80SS6
• Cytogenetics: 1 38.53 cM
• MW: 36.1 kDa