Mouse Sirt1 (NM_019812) AAV Particle

SKU MR227263A1V

AAV ORF Particle, Serotype AAV-2, Sirt1 (Myc-DDK-tagged) - Mouse sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae) (Sirt1), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Sirt1
• Synonyms: AA673258; S; Si; Sir2; Sir2a; Sir2alpha; SIR2L1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227263 representing NM_019812; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGACGAGGTGGCGCTCGCCCTTCAGGCCGCCGGCTCCCCTTCCGCGGCGGCCGCCATGGAGGCCG; CGTCGCAGCCGGCGGACGAGCCGCTCCGCAAGAGGCCCCGCCGAGACGGGCCTGGCCTCGGGCGCAGCCC; GGGCGAGCCGAGCGCAGCAGTGGCGCCGGCGGCCGCGGGGTGTGAGGCGGCGAGCGCCGCGGCCCCGGCG; GCGCTGTGGCGGGAGGCGGCAGGGGCGGCGGCGAGCGCGGAGCGGGAGGCCCCGGCGACGGCCGTGGCCG; GGGACGGAGACAATGGGTCCGGCCTGCGGCGGGAGCCGAGGGCGGCTGACGACTTCGACGACGACGAGGG; CGAGGAGGAGGACGAGGCGGCGGCGGCAGCGGCGGCGGCAGCGATCGGCTACCGAGACAACCTCCTGTTG; ACCGATGGACTCCTCACTAATGGCTTTCATTCCTGTGAAAGTGATGACGATGACAGAACGTCACACGCCA; GCTCTAGTGACTGGACTCCGCGGCCGCGGATAGGTCCATATACTTTTGTTCAGCAACATCTCATGATTGG; CACCGATCCTCGAACAATTCTTAAAGATTTATTACCAGAAACAATTCCTCCACCTGAGCTGGATGATATG; ACGCTGTGGCAGATTGTTATTAATATCCTTTCAGAACCACCAAAGCGGAAAAAAAGAAAAGATATCAATA; CAATTGAAGATGCTGTGAAGTTACTGCAGGAGTGTAAAAAGATAATAGTTCTGACTGGAGCTGGGGTTTC; TGTCTCCTGTGGGATTCCTGACTTCAGATCAAGAGACGGTATCTATGCTCGCCTTGCGGTGGACTTCCCA; GACCTCCCAGACCCTCAAGCCATGTTTGATATTGAGTATTTTAGAAAAGACCCAAGACCATTCTTCAAGT; TTGCAAAGGAAATATATCCCGGACAGTTCCAGCCGTCTCTGTGTCACAAATTCATAGCTTTGTCAGATAA; GGAAGGAAAACTACTTCGAAATTATACTCAAAATATAGATACCTTGGAGCAGGTTGCAGGAATCCAAAGG; ATCCTTCAGTGTCATGGTTCCTTTGCAACAGCATCTTGCCTGATTTGTAAATACAAAGTTGATTGTGAAG; CTGTTCGTGGAGACATTTTTAATCAGGTAGTTCCTCGGTGCCCTAGGTGCCCAGCTGATGAGCCACTTGC; CATCATGAAGCCAGAGATTGTCTTCTTTGGTGAAAACTTACCAGAACAGTTTCATAGAGCCATGAAGTAT; GACAAAGATGAAGTTGACCTCCTCATTGTTATTGGATCTTCTCTGAAAGTGAGACCAGTAGCACTAATTC; CAAGTTCTATACCCCATGAAGTGCCTCAAATATTAATAAATAGGGAACCTTTGCCTCATCTACATTTTGA; TGTAGAGCTCCTTGGAGACTGCGATGTTATAATTAATGAGTTGTGTCATAGGCTAGGTGGTGAATATGCC; AAACTTTGTTGTAACCCTGTAAAGCTTTCAGAAATTACTGAAAAACCTCCACGCCCACAAAAGGAATTGG; TTCATTTATCAGAGTTGCCACCAACACCTCTTCATATTTCGGAAGACTCAAGTTCACCTGAAAGAACTGT; ACCACAAGACTCTTCTGTGATTGCTACACTTGTAGACCAAGCAACAAACAACAATGTTAATGATTTAGAA; GTATCTGAATCAAGTTGTGTGGAAGAAAAACCACAAGAAGTACAGACTAGTAGGAATGTTGAGAACATTA; ATGTGGAAAATCCAGATTTTAAGGCTGTTGGTTCCAGTACTGCAGACAAAAATGAAAGAACTTCAGTTGC; AGAAACAGTGAGAAAATGCTGGCCTAATAGACTTGCAAAGGAGCAGATTAGTAAGCGGCTTGAGGGTAAT; CAATACCTGTTTGTACCACCAAATCGTTACATATTCCACGGTGCTGAGGTATACTCAGACTCTGAAGATG; ACGTCTTGTCCTCTAGTTCCTGTGGCAGTAACAGTGACAGTGGCACATGCCAGAGTCCAAGTTTAGAAGA; ACCCTTGGAAGATGAAAGTGAAATTGAAGAATTCTACAATGGCTTGGAAGATGATACGGAGAGGCCCGAA; TGTGCTGGAGGATCTGGATTTGGAGCTGATGGAGGGGATCAAGAGGTTGTTAATGAAGCTATAGCTACAA; GACAGGAATTGACAGATGTAAACTATCCATCAGACAAATCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227263 representing NM_019812; Red=Cloning site Green=Tags(s); ; MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPA; ALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLL; TDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDM; TLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFP; DLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQR; ILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKY; DKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYA; KLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLE; VSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGN; QYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPE; CAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_019812
• ORF Size: 2211 bp
• Buffer: PBS with 0.001% Pluronic F4251
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_019812.2
• RefSeq Size: 3920 bp
• RefSeq ORF: 2214 bp
• Locus ID: 93759
• UniProt ID: Q923E4
• Cytogenetics: 10 B4
• MW: 80.8 kDa