Mouse Rbak (NM_021326) AAV Particle

SKU MR223372A1V

AAV ORF Particle, Serotype AAV-2, Rbak (Myc-DDK-tagged) - Mouse RB-associated KRAB repressor (Rbak), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Rbak
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223372 representing NM_021326, codon optimized.; Due to the complexity of NM_021326, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTAGAAGCAAGGGCCCTTTGAGCTTCAAAGATGTGGCCGTCGCCTTTTCTCAGGAGGAGTGGCAGC; AGCTCGATCCCGAAGAAAGAACTACCTATAGGGACGTGATGCTCGAGACATACAGCAATCTGGTGTCAGT; CGGATACGACGTGACTAAGCCTAATATGATCATCAAACTGGAGCAGGGCGAGGAACCTTGGACTGTCGAG; GGGGACAGGCACGCGCAAAGACATCTCGAAATTAGCAAGGTCTACGACCCACGGGAAGGGATAGAAGAAA; TCGGAGAAAAGCACCTCCAGTGTGACGACGATCCCTACTGTTGGAGGGCTGAAAAGGGTGCCGCCTTCGA; CGAGGCCTATACCCTGGAGACGGCCCTTATTTCCCCCTCCAGTGGCGCACATTCCTGTGTCAGTTGCGGT; GAGACACTGGAGAGCGTGAGTGAACTGATTAGCTCCGACGGGTCTTATGCTTTGGAAAAGCCTAGCATGT; GCTTCGAGTGCGGCAAGGCCTATGGCGAGTCCCTGGAAGACTTCAACCAGGACGAAGGGAACTCATCACA; GCACGATGAAAATATCCTCCAGAAAGTGACCATCTTGGAAAAGCCTTTTGCCTATGAGTGTATGGAAGCA; CTGGATAGTGAGTCTGTGTTCATGGCGCGGGAGCGGGCATACATGGGCGAGAAGCCCTATGATTGGGGCG; ACTCAGGACCTGATTTTATTCAGATGAGCGATTTTTCAACCTACCCTAGGAGTCAGATGGAACTGAAACC; TTTCGAATGCACTCAATGTGGAAAGAGCTTTTGTAAGAAAAGCAAGTTTATCATCCACCAGAGAGCTCAT; ACAGGAGAGAAGCCCTACGCCTGTTCTGTCTGTGGAAAGTCTTTCTCTCAGAAGGGCACGCTGACCGTGC; ACCGCCGGAGCCATCTGGAGGAGAAGCCCTACAAGTGTAATGAATGCGGCAAGACCTTCTGTCAGAAGCT; TCATTTGACGCAGCATCAGCGAACGCACAGTGGCGAGAAGCCTTACGAATGTTCAGAGTGTGGTAAAAGC; TTCTGTCAGAAGACGCACCTGACATTGCATCAGCGCAACCACTCCGGCGAGCGGCCCTACCCGTGTAACG; AGTGCGGCAAGTCCTTCTCCCGCAAGTCAGCCCTCAACGACCATCAGAGAACACATACCGGCGAAAAGCT; CTACAAGTGTAATGAGTGTGGCAAAAGTTATTATCGGAAAAGCACCCTGATTACACACCAGCGGACTCAT; ACAGGCGAAAAACCGTATCAGTGCAGCGAGTGTGGTAAATTCTTCTCCAGAGTGTCTTATCTGACCATTC; ACTATAGAAGCCACCTGGAGGAGAAACCCTACGAGTGCACTGAGTGTGGCAAAACATTTAATCTGAACTC; CGCCTTTATCAGACACTGGAAGGTGCATGCCGAGGAAAGAGTGCAAGAGTGCGGAGAGTGTGGGAAACCC; TCCCCTCTGCAGTGCGCACCTGATCATACTGGCGACCTCGGGGAAAAACGGTATGAATGTAACGAGTGCG; GAAAAACATTCCTGGACTCCAGCGCTTTTCACCGCCATCAGTCAGTCCCTGAGGGTGAGAAAACCTACGA; ATGCAACATATGTGGCAAATCATTTAGTGATAGTAGCTGCTATACAGTCCACTATAGGGGCCACTCCGAG; GAGAAGCCTTTCGGATGTTCTGAGTGTGGAAAAACCTTCTCCCATAACTCTTCTCTGTTTCGACACCAGA; GGGTGCATACCGGGGAGAAGCCCTATGAGTGTTACGAATGTGGCAAATTCTTCTCTCAGAAGAGTTATCT; GACAATACATCACCGGATTCACAGTGGGGAGAAGCCCTACGAGTGCAGTAAGTGCGGTAAAGTGTTCAGC; CGAATGTCTAATCTGACGGTGCACTACAGATCTCACTCCGGAGAAAAGCCTTACGAGTGTAACGAGTGCG; GTAAAGTTTTCAGTCAGAAGAGCTATCTGACTGTCCACTACCGAACGCACTCAGGCGAAAAGCCATACGA; ATGTAACGAGTGTGGTAAAAAGTTTCACCATCGGTCTGCCTTTAATAGTCATCAGCGAATTCACAAGAGA; GGCACCGTTAACGTACTGACTGTTGAGAAACTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223372 representing NM_021326; Red=Cloning site Green=Tags(s); ; MSRSKGPLSFKDVAVAFSQEEWQQLDPEERTTYRDVMLETYSNLVSVGYDVTKPNMIIKLEQGEEPWTVE; GDRHAQRHLEISKVYDPREGIEEIGEKHLQCDDDPYCWRAEKGAAFDEAYTLETALISPSSGAHSCVSCG; ETLESVSELISSDGSYALEKPSMCFECGKAYGESLEDFNQDEGNSSQHDENILQKVTILEKPFAYECMEA; LDSESVFMARERAYMGEKPYDWGDSGPDFIQMSDFSTYPRSQMELKPFECTQCGKSFCKKSKFIIHQRAH; TGEKPYACSVCGKSFSQKGTLTVHRRSHLEEKPYKCNECGKTFCQKLHLTQHQRTHSGEKPYECSECGKS; FCQKTHLTLHQRNHSGERPYPCNECGKSFSRKSALNDHQRTHTGEKLYKCNECGKSYYRKSTLITHQRTH; TGEKPYQCSECGKFFSRVSYLTIHYRSHLEEKPYECTECGKTFNLNSAFIRHWKVHAEERVQECGECGKP; SPLQCAPDHTGDLGEKRYECNECGKTFLDSSAFHRHQSVPEGEKTYECNICGKSFSDSSCYTVHYRGHSE; EKPFGCSECGKTFSHNSSLFRHQRVHTGEKPYECYECGKFFSQKSYLTIHHRIHSGEKPYECSKCGKVFS; RMSNLTVHYRSHSGEKPYECNECGKVFSQKSYLTVHYRTHSGEKPYECNECGKKFHHRSAFNSHQRIHKR; GTVNVLTVEKL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_021326
• ORF Size: 2133 bp
• Buffer: PBS with 0.001% Pluronic F3571
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_021326.3, NP_067301.1
• RefSeq Size: 3480 bp
• RefSeq ORF: 2136 bp
• Locus ID: 57782
• UniProt ID: Q8BQC8
• Cytogenetics: 5 G2
• MW: 81.7 kDa