Mouse Fto (NM_011936) AAV Particle

SKU MR208064A1V

AAV ORF Particles, serotype AAV-2, Fto (Myc-DDK-tagged) - Mouse fat mass and obesity associated (Fto), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Fto
• Synonyms: AW743446; mKIAA1752
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208064 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGCGCGTCCAGACCGCGGAGGAACGAGAGCGGGAAGCTAAGAAACTGAGGCTCCTTGAGGAGCTTG; AAGACACTTGGCTTCCTTACCTGACCCCCAAAGATGATGAGTTCTATCAGCAGTGGCAGCTGAAATACCC; TAAACTGGTTTTCCGAGAGGCCGGCAGCATACCAGAGGAGCTGCATAAGGAGGTCCCCGAGGCCTTTCTC; ACACTGCATAAGCATGGCTGCTTGTTTCGGGACGTGGTGAGGATCCAAGGCAAAGATGTGCTCACCCCAG; TGTCTCGCATCCTCATCGGGGACCCAGGCTGCACCTACAAGTACTTGAACACCAGACTCTTCACGGTGCC; CTGGCCCGTGAAGGGCTGCACGGTCAAGTACACAGAGGCTGAGATCGCCGCTGCATGTCAGACCTTCCTA; AAGCTCAATGACTACCTCCAGGTGGAGACCATCCAGGCCTTGGAAGAACTGGCTGTCAGAGAGAAGGCCA; ATGAAGACGCTGTGCCACTGTGCATGGCAGAGTTCCCCAGGGCCGGCGTGGGGCCGTCCTGCGATGATGA; AGTGGACCTTAAGAGCAGAGCAGCCTACAACGTGACTTTGCTAAACTTCATGGATCCTCAGAAGATGCCC; TACTTGAAAGAGGAGCCCTATTTCGGCATGGGGAAGATGGCGGTGAGCTGGCATCACGATGAGAACCTGG; TGGACAGGTCAGCCGTGGCAGTGTACAGCTATAGCTGCGAAGGCTCTGAGGATGAAAGTGAGGACGAGTC; CAGCTTCGAAGGCAGAGATCCTGATACTTGGCATGTTGGTTTTAAGATCTCTTGGGACATCGAGACACCA; GGATTAACAATCCCTCTTCACCAGGGAGACTGCTATTTCATGCTGGATGACCTCAATGCCACCCACCAGC; ACTGTGTTTTGGCTGGCTCACAGCCTCGGTTTAGTTCCACTCACCGTGTGGCAGAGTGCTCAACAGGCAC; CTTGGATTATATCTTAGAACGCTGTCAGTTGGCGCTGCAGAATGTCCTCAATGACTCAGACGATGGCGAC; GTCTCGTTGAAATCCTTTGATCCTGCAGTTTTGAAACAAGGAGAGGAAATCCATAATGAGGTGGAGTTTG; AGTGGCTGAGGCAGTTCTGGTTTCAAGGCAATCGATACAAACTTTGCACCGATTGGTGGTGTGAGCCCAT; GACTCACCTGGAGGGGCTGTGGAAGAAGATGGAGAGCATGACAAATGCGGTGCTCCGTGAAGTTAAAAGA; GAGGGGCTCCCGGTGGAACAAAGGAGTGAGATTCTGTCTGCCATCCTGGTCCCGCTCACCGTGCGCCAGA; ACCTGAGGAAGGAGTGGCATGCCAGGTGCCAGTCCCGAGTCGTCCGGACTTTACCAGTACAGCAGAAACC; AGACTGCCGGCCATATTGGGAGAAGGATGACCCTTCCATGCCTCTGCCCTTTGACCTCACAGACGTGGTT; TCCGAGCTCAGAGGCCAGCTGCTGGAAGCAAGATCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208064 protein sequence; Red=Cloning site Green=Tags(s); ; MKRVQTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLVFREAGSIPEELHKEVPEAFL; TLHKHGCLFRDVVRIQGKDVLTPVSRILIGDPGCTYKYLNTRLFTVPWPVKGCTVKYTEAEIAAACQTFL; KLNDYLQVETIQALEELAVREKANEDAVPLCMAEFPRAGVGPSCDDEVDLKSRAAYNVTLLNFMDPQKMP; YLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGSEDESEDESSFEGRDPDTWHVGFKISWDIETP; GLTIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTGTLDYILERCQLALQNVLNDSDDGD; VSLKSFDPAVLKQGEEIHNEVEFEWLRQFWFQGNRYKLCTDWWCEPMTHLEGLWKKMESMTNAVLREVKR; EGLPVEQRSEILSAILVPLTVRQNLRKEWHARCQSRVVRTLPVQQKPDCRPYWEKDDPSMPLPFDLTDVV; SELRGQLLEARS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_011936
• ORF Size: 1506 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_011936.1
• RefSeq Size: 3586 bp
• RefSeq ORF: 1509 bp
• Locus ID: 26383
• UniProt ID: Q8BGW1
• Cytogenetics: 8 44.34 cM
• MW: 58 kDa