Mouse Nle1 (NM_145431) AAV Particle

SKU MR207771A1V

AAV ORF Particles, serotype AAV-2, Nle1 (Myc-DDK-tagged) - Mouse notchless homolog 1 (Drosophila) (Nle1), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Nle1
• Synonyms: AL022765; BC018399; l11Jus1; l11Jus4; Nle
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207771 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCGGCGGTGGTGGAAGAGGCGGCGGCAGGCGACGTGCAGCGGCTGCTCGTACAGTTCCAGGATG; AGGGCGGTCAGCTGCTGGGCTCCCCGTTCGACGTGCCCGTGGACATCACGCCGGACAAGCTGCAGCTGGT; GTGCAACGCGCTGCTGGCCCAGGAGGAGCCCCTGCCCCTGGCGTTCTATGTCCACGATGCTGAGATTGTG; TCCTCCCTGGGGAAGACGCTGGAGTCCCAGTCTGTGGAGACAGAGAAAATCGTCGATATCATCTACCAGC; CCCAAGCTGTCTTCAGGGTCCGGGCTGTGACGCGGTGCACCAGCTCATTGGAGGGTCACAGCGAAGCTGT; CATTTCTGTGGCCTTCAGCCCCACAGGAAAGTACCTGGCCAGTGGTTCCGGAGACACCACTGTGCGCTTC; TGGGATCTCAGCACTGAGACACCGCACTTCACATGCAAAGGACACCGGCACTGGGTCCTTAGCATATCCT; GGTCCCCAGATGGCAAGAAACTGGCCTCAGGCTGCAAGAATGGCCAGATTCTGCTGTGGGACCCAAGCAC; GGGGCTGCAGGTGGGCAGGACCCTCACTGGCCACAGCAAATGGATCACAGGCCTGAGCTGGGAGCCCCTT; CACATGAACCCCGAGTGCCGCTACGTGGCCAGCAGCTCCAAAGACGGCAGTGTACGAGTCTGGGATACAA; CCGCAGGCCGCTGTGAACGCATCCTCACGGGACACACGCAGTCAGTCACCTGCCTCCGATGGGGAGGAGA; TGGGCTTCTATATTCTGCTTCCCAGGACCGAACCATCAAAGTCTGGAGGGCCCATGACGGTGTGCTGTGT; CGTACTCTTCAAGGTCATGGCCACTGGGTGAACACCATGGCGCTTAGCACAGACTATGCCCTGCGCACAG; GGGCCTTTGAGCCTGCTGAAGCCACAGTGAATGCCCAAGACCTGCAGGGGTCCTTGAAGGAGCTGAAGGA; GAGGGCATCGAGCCGCTACAACCTCGTGCGGGGCCAAGGCCCAGAGAGGCTGGTCTCTGGCTCAGACGAC; TTCACCTTATTCCTGTGGTCCCCAGCAGAGGACAAGAAGCCCCTCGCCCGGATGACAGGACACCAGGCCC; TCATCAACCAGGTGCTCTTCTCTCCTGACTCGCGCATCGTGGCCAGTGCCTCCTTCGACAAGTCTATCAA; GCTGTGGGATGGCCGGACGGGCAAGTACCTAGCTTCCCTGCGTGGCCACGTGGCAGCCGTGTACCAGATT; GCCTGGTCAGCTGACAGCCGACTATTAGTCAGCGGCAGCAGTGACAGCACGCTGAAGGTGTGGGATGTGA; AGGCCCAGAAACTGGCCACGGACCTGCCTGGCCATGCCGATGAGGTATATGCTGTTGACTGGAGTCCAGA; TGGCCAGAGAGTAGCAAGCGGCGGGAAAGACAAGTGCCTCCGGATATGGAGAAGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207771 protein sequence; Red=Cloning site Green=Tags(s); ; MAAAVVEEAAAGDVQRLLVQFQDEGGQLLGSPFDVPVDITPDKLQLVCNALLAQEEPLPLAFYVHDAEIV; SSLGKTLESQSVETEKIVDIIYQPQAVFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRF; WDLSTETPHFTCKGHRHWVLSISWSPDGKKLASGCKNGQILLWDPSTGLQVGRTLTGHSKWITGLSWEPL; HMNPECRYVASSSKDGSVRVWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLC; RTLQGHGHWVNTMALSTDYALRTGAFEPAEATVNAQDLQGSLKELKERASSRYNLVRGQGPERLVSGSDD; FTLFLWSPAEDKKPLARMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQI; AWSADSRLLVSGSSDSTLKVWDVKAQKLATDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_145431
• ORF Size: 1455 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_145431.1, NP_663406.1
• RefSeq Size: 1771 bp
• RefSeq ORF: 1458 bp
• Locus ID: 217011
• UniProt ID: Q8VEJ4
• Cytogenetics: 11 C
• MW: 53.1 kDa