Mouse Nim1k (NM_175538) AAV Particle

SKU MR206958A1V

AAV ORF Particles, serotype AAV-2, Nim1 (Myc-DDK-tagged) - Mouse RIKEN cDNA E130304F04 gene (E130304F04Rik), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Nim1k
• Synonyms: E130304F04Rik; Nim1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206958 representing NM_175538; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACAGCAGTGTTTGTGAATGGAGGCGGCCTGGTGAACACACATTGTGACAGGTGGGAGCGGAGGGACA; GCGTGGAAAGCAGCTGTCAGACCGAGGGCGGCAAGGACAGCGAGGAGGACCAGCAGCGCCAGCTGACACC; CTTCGAGAAACTGACTCAGGACATGTGCCAGGATGAGAAGGTGGTGAGGGAGATCACGCTGGGGAAACGC; ATTGGCTTCTATCGGATTCGAGGGGAGATCGGAAGCGGAAACTTTTCCCAGGTCAAGCTGGGGATTCACT; CCCTAACCAAAGAAAAGGTGGCCATTAAGATTCTGGACAAAACCAAGTTAGACCAGAAAACCCAAAGGCT; GTTATCCAGAGAGATTTCCAGCATGGAAAAGTTGCATCATCCCAACATCGTCCGTCTTTATGAAGTCGTG; GAGACACTGTCCAAGCTACACCTAGTGATGGAGTATGCAGGAGGTGGGGAGCTTTTTGGAAAAATTAGCA; CCGAGGGGAAGCTTTCTGAACCGGAAAGCAAGCTTATCTTCTCCCAGATCCTGTCTGCCGTGAAGCACAT; GCATGAAAATCAAATTATTCACAGAGATCTGAAAGCAGAAAACGTCTTTTATACCAGTAGGACTTGTGTG; AAGGTGGGAGATTTTGGATTCAGCACAGTAAGTAAGAAAGGCGAGATGCTGAACACTTTCTGTGGATCTC; CACCCTACGCGGCACCAGAACTCTTTCGAGACCAACACTACGTTGGTGTTTATGTGGATATCTGGGCCTT; GGGTGTCCTTTTGTACTTCATGGTGACTGGCACGATGCCATTTCGAGCGGAGACCGTGGCCAAGCTAAAG; AAGAGCATCCTCGAGGGTACCTACACCATACCCCAGCATGTGTCTGAGCCTTGTCACCGGCTCATCCGAG; GAGTCCTTCAGCCCACGCCCACAGAGAGGTATGGGATCAACTACATCATGAGCAATGAATGGATGCGAGG; GGTGCCATACCCCACCCCTTTGGAACCGTTCCAACTGGATCCCAAACATTTGTCGGAAACCAGTACCCTC; AAAGAAGAAGAAAACGAGGTGAAGAGCACTTTAGAACACTTGGGGATTACAGACGAACATATCCGGAATA; ACCAAGGGAGAGATGCTCGGAGCTCTATCACGGGGGTCTATAGAATCATTTTACATCGAGTGCAAAGAAG; AAAAGCATTGGAAAGTGTGCCAATAATGATACTACCAGAACCTAAAGAAAGGGACTTAAAGAAAGGGTCC; CGTATCTACAGAGGCATAAGACACACATCTAAATTTTGTTCAATTTTA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206958 representing NM_175538; Red=Cloning site Green=Tags(s); ; MTAVFVNGGGLVNTHCDRWERRDSVESSCQTEGGKDSEEDQQRQLTPFEKLTQDMCQDEKVVREITLGKR; IGFYRIRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIVRLYEVV; ETLSKLHLVMEYAGGGELFGKISTEGKLSEPESKLIFSQILSAVKHMHENQIIHRDLKAENVFYTSRTCV; KVGDFGFSTVSKKGEMLNTFCGSPPYAAPELFRDQHYVGVYVDIWALGVLLYFMVTGTMPFRAETVAKLK; KSILEGTYTIPQHVSEPCHRLIRGVLQPTPTERYGINYIMSNEWMRGVPYPTPLEPFQLDPKHLSETSTL; KEEENEVKSTLEHLGITDEHIRNNQGRDARSSITGVYRIILHRVQRRKALESVPIMILPEPKERDLKKGS; RIYRGIRHTSKFCSIL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_175538
• ORF Size: 1308 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_175538.3, NP_780747.1
• RefSeq Size: 4103 bp
• RefSeq ORF: 1311 bp
• Locus ID: 245269
• UniProt ID: Q8BHI9
• Cytogenetics: 13 D2.3
• MW: 50.2 kDa