IRAK4 (NM_001114182) Human 3' UTR Clone

CAT#: SC219805

3' UTR clone of interleukin-1 receptor-associated kinase 4 (IRAK4) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IRAK4
• Synonyms: IMD67; IPD1; IRAK-4; NY-REN-64; REN64
• ACCN: NM_001114182
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC219805 3’UTR clone of NM_001114182
  The sequence shown below is from the reference sequence of NM_001114182. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGCTGCTGCAAGAGATGACAGCTTCTTAAAACTTTATTGGAAAAGACTCTTGACTTTTTATATACACC
  TATCTCAACCATTTTTTTAACTGATTTTTTTCCTAAATATTCTTCTTTACCTTTAACAAGGCATAGGCT
  GTTGCAGGACAGTGGTTATTAAAGCATGGGTTGAACTTCCAAAATATAAAAATAGAGCCACCATATCAA
  CACTTAGCCCTACCCATTAGTATCACCCCCAGTTCTTACAGTAATCCCTGAGAAATCTCCTTCAAGCAT
  CACCAAACACAGTTTGAAAATTACAGGGTTAGCAAAAAGAGCCTGGGCTGTATGTAGGGTGGAAACACT
  CTGATCTGAAGCCCAGCTGACTCCACTACTAATTTGCTGTAAAGCTTTGGACATACACTTAGCTGCTGT
  GAGCCACTAATAACATTGGGCTAATATCTGCTGTGCTTCTCTGACAGGTAGTCATGAAAATCAAATGAT
  GCAAAATATATACAAGCACTTTGTAAATTGTAAAATGATACAAAATTTAAAGTTTATAGAGCCAGTTAC
  AAAATCCTATTAGTCATATATTTATAGATTGTGTTCACAGCAATCATTTAACCACAAATAAAATATCCC
  TTGATGATACTGCCATAATGATATGTCCATTATTAGATTATGTTACATGACAAAGTTGAAGGAATTTGG
  CAGATGCAGTTAAGGTTCCTAAACAACTCACTTTGAGACTGTTGAAAGGGCCTGACCTAATCAAGTGAA
  CCCTTGCAAGAAGAATTCTCCTTGTAAGCCTTGAAGAAGTATGTGAGAGGGCCACATTGGCTAAAACCT
  AAAGGTGGCCTCTAGGAGATGAGACCTACCTTCCAGTTGTCAGCAAGCAGGAAAAAAAAATTGGGACCT
  CAGTTGCAACCACAAGGAACTGAATTCTGCCAAAAATCTGAGTCAGCTTAGAAGAGTACTCCAAGCTTC
  AGATGATAACCACAGCCTGGGCTGACACCTGGATTTCAGCTTTGCATGATCCTCAGTATGAGAATCTAT
  CTGTTCTGTGCTGGACTTCTAATATATAGAACTGTGAGATAATGGGTCACATTGGCTGGATGTGGTGGC
  TCATACCTGTAAATCCCAGCACTTTGGGAGGCCGAGGCAGGCAGATCACCTGAGGTCAAGAGTTCAAGA
  CCGGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGACGAGCGTGGTGGTGG
  ACACCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGACTAGCTGGAACCAGGGAGGTAGAGGTTG
  CAGTGAGCTGAGATCGTGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCATCATAAATAAATA
  AATAAATAAATGGGTCACATTAAGCCTTTAAGTTTGTGGTAATTTATTATTCAGTAATAGAAAACAAAT
  ACAGATACTCTCCCATGATGTTTTTCCCATGATGATTTCCCATGATATTTACAGGTTTTGCCCACATTT
  GAGGGGTATGTGGAAATTATACAGAGCATGTACAGCGGGAGGCTTATAGTGTACGTACTGAAATGTGGG
  GTTGGAGCCCCAACACAGAGACCCCAGCAGGACACTGCCTAGTAGAGCTATGGGAAGGGTGCTGCCACC
  CTCCAGACTTGAGAATTGTAGAGCCACCAGCAGCTTGCACTCTGAGCTTGGAAAAGCCACAGGCACTCA
  ACTTCAACCATGAGGGAAGCCACGCACCCTGCAAAGCCACAGGAGTGGAGCTGCCCACGGCCTCGAGGG
  CCCACCCCTTGCACCAGTGTGCCAGGATGTGGGACATGGAATCAAGGAATATGTTAGGGCTTTTTTTTT
  TTTTTTTTTGAGACGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCACGATCTCGGCTCACTG
  CAAGCTCTGCCTCCCAGGTTCACGCCATTCCCCTGCCTCAGCCTCCCCAGTAGCTGGGACTACAGGTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001114182.3
• Summary: This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
• Locus ID: 51135
• MW: 75.8