ACAT1 (ACACA) (NM_198834) Human 3' UTR Clone

SKU SC218496

3' UTR clone of acetyl-Coenzyme A carboxylase alpha (ACACA) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: ACAT1
• Synonyms: ACAC; ACACAD; ACC; ACC1; ACCA
• ACCN: NM_198834
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC218496 3’UTR clone of NM_198834 ; The sequence shown below is from the reference sequence of NM_198834. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CTCTCCACAATGGATTCCCCTTCCACGTAGGAAGAGCTTCCTGCCTGTCCCTGCCCTGTCTCTGGAGAA; AAGGGCTAGAGCTGCCTTTTACAACTGTAACCACTGTAATGAGAAGGCACAGGAGACCCAGCACTGGAG; TCAAATGGCATTTTACTTCCTCTCGTTTCAGGTTATGCATGACATCCTGGGATGTAAGATCACAGAATC; CCCCTCCAGCCCACCAGTCACACCTACCCCATTCAGTATTTATTACCCTGGCCAGGCCTAGTCCTCCAC; TCCCTGCACAGGACTGAGAAGGCAATGAAAGGTACAAACATGTACCATGAGGTCTTACTAACCAAAGTA; GGGCTGCCCCTCCTGTCCTGACAGCCCCTTGGCCTCCCAGCATGGGGAAGCGTGAGGAGTTGCCCAGCA; GTGAGCAGCCCCCCTCACTCCTGGCCCCATGAGCCGCAGCCACAGGCAGCAGAGGAGGGCTAAGGAGAG; GAGGAAGCCTCAAGTCCATTGTTTATTACCCCGACTCTTAGCCCAGCACACAGTAGGCACTGGAGAGGA; ATGATTCCCAGTTTAACCACACTACGGTACCTTTTATGAAGAAAAATTAGAGCATAAAATCTACTACAA; GCTCCATAGGAACTCAAAGATGAGGGCAAAACTGTGAGCCAAGAAGCAGAGAAAGAAAATAGAACCAGT; TATTCTTGATTTAGGGGACCTCAACCTTGGGTTCAGTCTCTGAGGACAAAGGGAAAGGTAGTTGTTGGC; CTGCCTCTCGCCTGCACGTCACTGCTGGACTAGCTGTCGCATGTGGCTGGGAGCTGCAAGGCCAGTGCT; TGAGGGGCCCCAGCAGTTCCACAGGTGGTGAAGCCTGAGTTGGCAGAGGAGGAGCCAGAAGAGAACTGC; CCTTTCTGCACTGGTGGAAACTAGTTATTTATGCCATGTGGAGAGCCAGTGAGATAGATAGATAGTCTG; TTTGTTTTGAGGACTTGGAAAGTTGTTCCTATGAAGCCTGGAGCTTGGATGGTTTTGAGAGGTTAATGG; TGCCTCCACACTCACTCTTCCCTAGTTCCAGGATTACTGTCCTAGCAGCTAACGGTTCTACTCTCTTCC; CCAGAGTGTAGACAGGCAGCAGGTCTCCCCACAGCTCTGAAAGGACCCTGGTGACAGCTACACCCTCAG; CACCAGGAGCTGGCCTTCCTGATGAGGGAGGCTTCCAGGAAACACAGAATCCACATGACCTTAAGATTA; TTTACAACTCAGTCATGGTGCTGCTGTCCTCCAGGCTTACTGGCCCCTCCTGACTGGCATCAGGGGCTT; CCTCAGGTGGTGGAGAGAGTTTACTTTCAACAACTAGTTTATTCAAGAAAAGAACTTACTGATTCCTCT; GTTCCTAAAGCAAGAGTGGCAGGTGATCAGGGCTGGTGTAGCATCCGGTTCCTTTAGTGCAGCTAACTG; CATTTGTCACTGATGACCAAGGAGGAAATCACTAAGACATTTGAGAAGCAGTGGTATGAACGTTCTTGG; ACAAGCCACAGTTCTGAGCCTTAACCCTGTAGTTTGCACACAAGAACGAGCTCCACCTCCCCTTCTTCA; GGAGGAATCTGTGCGGATAGATTGGCTGGACTTTTCAATGGTTCTGGGTTGCAGGTGGGCACTGTATGG; CTGGGTATGGAGCGGACAGCCCCCAGGAGTCAGAGCCTCAGCCCGGCTGCCCTGGTGGAAGGTACAGGT; GTTCAGCACCTTCAGAAAAGGGCATAAAGTGGTGGGGGACAATTCTCAGTCCAGGAAAATGCATTGACC; ATTGCTGGCTATTTGCTTACCTAGTAAGAATTGGATTCATTTTTGACCAGATTATTCTTCTATGCTTTT; TTGCAATAAATCAAATCCCACATATCTACAAGTGGTATGAAGTCCTGCACCCCCCAGGAGGCCTGTCCA; GGCATGTCTTCAGAGGCAGGGTGGGTTACACTCATTTACCTCCCCTCTCCCCACCAAATTATGACACA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198834.3
• Locus ID: 31
• Summary: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]