BMI1 (NM_005180) Human 3' UTR Clone

CAT#: SC217171

3' UTR clone of BMI1 polycomb ring finger oncogene (BMI1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: BMI1
• Synonyms: flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
• ACCN: NM_005180
• Insert Size: 1972 bp
• Sequence Data:
  Insert Sequence

  >SC217171 3’UTR clone of NM_005180
  The sequence shown below is from the reference sequence of NM_005180. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AATGGGTCATCAGCAACTTCTTCTGGTTGATACCTGAGACTGTTAAGGAAAAAAATTTTAAACCCCTGA
  TTTATATAGATATCTTCATGCCATTACAGCTTTCTAGATGCTAATACATGTGACTATCGTCCAATTTGC
  TTTCTTTTGTAGTGACATTAAATTTGGCTATAAAAGATGGACTACATGTGATACTCCTATGGACGTTAA
  TTGAAAAGAAAGATTGTTGTTATAAAGAATTGGTTTCTTGGAAAGCAGGCAAGACTTTTTCTCTGTGTT
  AGGAAAGATGGGAAATGGTTTCTGTAACCATTGTTTGGATTTGGAAGTACTCTGCAGTGGACATAAGCA
  TTGGGCCATAGTTTGTTAATCTCAACTAACGCCTACATTACATTCTCCTTGATCGTTCTTGTTATTACG
  CTGTTTTGTGAACCTGTAGAAAACAAGTGCTTTTTATCTTGAAATTCAACCAACGGAAAGAATATGCAT
  AGAATAATGCATTCTATGTAGCCATGTCACTGTGAATAACGATTTCTTGCATATTTAGCCATTTTGATT
  CCTGTTTGATTTATACTTCTCTGTTGCTACGCAAAACCGATCAAAGAAAAGTGAACTTCAGTTTTACAA
  TCTGTATGCCTAAAAGCGGGTACTACCGTTTATTTTACTGACTTGTTTAAATGATTCGCTTTTGTAAGA
  ATCAGATGGCATTATGCTTGTTGTACAATGCCATATTGGTATATGACATAACAGGAAACAGTATTGTAT
  GATATATTTATAAATGCTATAAAGAAATATTGTGTTTCATGCATTCAGAAATGATTGTTAAAATTCTCC
  CAACTGGTTCGACCTTTGCAGATACCCATAACCTATGTTGAGCCTTGCTTACCAGCAAAGAATATTTTT
  AATGTGGATATCTAATTCTAAAGTCTGTTCCATTAGAAGCAATTGGCACATCTTTCTATACTTTATATA
  CTTTTCTCCAGTAATACATGTTTACTTTAAAGATTGTTGCAGTGAAGAAAAACCTTTAACTGAGAAATA
  TGGAAACCGTCTTAATTTTCCATTGGCTATGATGGAATTAATATTGTATTTTAAAAATGCATATTGATC
  ACTATAATTCTAAAACAATTTTTTAAATAAACCAGCAGGTTGCTAAAAGAAGGCATTTTATCTAAAGTT
  ATTTTAATAGGTGGTATAGCAGTAATTTTAAATTTAAGAGTTGCTTTTACAGTTAACAATGGAATATGC
  CTTCTCTGCTATGTCTGAAAATAGAAGCTATTTATTATGAGCTTCTACAGGTATTTTTAAATAGAGCAA
  GCATGTTGAATTTAAAATATGAATAACCCCACCCAACAATTTTCAGTTTATTTTTTGCTTTGGTCGAAC
  TTGGTGTGTGTTCATCACCCATCAGTTATTTGTGAGGGTGTTTATTCTATATGAATATTGTTTCATGTT
  TGTATGGGAAAATTGTAGCTAAACATTTCATTGTCCCCAGTCTGCAAAAGAAGCACAATTCTATTGCTT
  TGTCTTGCTTATAGTCATTAAATCATTACTTTTACATATATTGCTGTTACTTCTGCTTTCTTTAAAAAT
  ATAGTAAAGGATGTTTTATGAAGTCACAAGATACATATATTTTTATTTTGACCTAAATTTGTACAGTCC
  CATTGTAAGTGTTGTTTCTAATTATAGATGTAAAATGAAATTTCATTTGTAATTGGAAAAAATCCAATA
  AAAAGGATATTCATTTAGAAAATAGCTAAGATCTTTAATAAAAATTTGATATGAAAAGCACAATGTGCA
  GAAGTTATGGAAAACCTATAGAGGATTACAACAGGTAAACGTTAAAGAGAATACATTGCTGACTTATAG
  TGATGTGGCTAAGAAGTACATGCTTTGTTGTAAAATTGCTTGAAAGCCCATTGAAAGATGTATCTGTTT
  ATTTACAGTCTTTGAAGTAAAAGTTACCAATGTTTGCCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005180.9
• Summary: This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]
• Locus ID: 648
• MW: 76.2