Prostaglandin dehydrogenase 1 (HPGD) (NM_000860) Human 3' UTR Clone

SKU SC216485

3' UTR clone of hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Prostaglandin dehydrogenase 1
• Synonyms: 15-PGDH; PGDH; PGDH1; PHOAR1; SDR36C1
• ACCN: NM_000860
• Insert Size: 1811 bp
• Sequence Data:
  Insert Sequence

  >SC216485 3’UTR clone of NM_000860 ; The sequence shown below is from the reference sequence of NM_000860. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; ACAACTCCATTTCAAGCAAAAACCCAATGAACAGCTTATGTGTTAGCCATAGCTGAAAATAAGCACAAA; TAGCTTATATTCAGATCCTATCTTCATTTGAATATAGCTTTTAAATGAAATGTTACAGTTTGAAGTTTT; CCTTCATGCACTTGGTGATAAACGTTTTCTAAATTTTTAGTTAAGTATATGGATAAAAAGTTATGAACT; ATTAAAAATGTGATGTGGACCAAAGGCTAGGTTGTAATCTTGATAGTCTAAAAAATGATCAAAACAAAT; GATTTTCAAGGAATATTCAATATTCTGCCTTTCAGAAAGTGTATTTATATCTGTGCTTCATAAATATTA; ATGTTCTTCAGAACATCATTTTAAAGGAGATACTTGAATTGTTATTTAAATCAAACCAGATGTAAAACA; CTCACATACAAGTTCATACTTTAAAAGAGGAAAGCTACTTAACAATGACAAATATTTCACAATAATAAT; TTTTACTTATATACCATCTTTCAACTGAACATTTCAGTTCTTCCAAGAGCTTCTTAGAGTAGTATATTT; TGGGGGCAGTCAAGGAATAAACTACAGTGTAAACATATCCCAGATGAAAACTGCTGTATGGAAAAATGA; CAGAAAGTAACTGATTGACACTGTTGATTCACAGTTCAGCCTCCTATCTGGGAAAGACATTTCTTTCCT; CTGCTCACTTTAAGAACTTTTACCGACTCCAAAAATCTCAGGAATTAAACTTTTAACAGTTACAGCAAT; AAAGAATAGTTAGTACTCCAAAAATATTATATTTAAGATGCTCAACAAGAAAAAAATGCAAATGTAATA; TTTTTTTCAAATTACTTCTTTATTGACTTGTCCAAATTTCAAAAGTGCCTACCCTTCAATAAAACTTTT; TTATTCTGATCTCCATAAATTACTTAGTCTTCTATGTATAGCTATCAAGGAAATAAAACCAATTTTGCC; ACAGCCACAACTGTAAATGTTTTTGTACCCATGCTGAAACTCATAACAACACAGACATAAAAATAGCTG; TGAGGTTTTGCTTTTTTTGTTGTCAGCTATCTTAAGAATCATTAAATACACCTGCTTTGGGTAAAACTC; TTTGCAAGCAGTAATTAACACTAGTAACAGTGAAAGCACAAGATTTCCAAATCAGTCGTTTTCTCAAAA; AAATATCGTATAAGTGACTCATCCTGTCTGCTAACTCCAGACCTCCCAGCTTGAAGCCAAATCTTTCCA; TGTGAGATTGATATGGATTTCCTAGAAGTACTGGAATGTTGTCATATCTTGCCCTATTTTAATTCTGCT; ATAGAAAACAATTGCCTTCACTTTTAAGGAGTAATTTGAATATTAATAACTCTGGTCTAGATTTTCATA; TAATGTATTAAAGACAAAGTAGTGAACATCAATGAACATCTGATAGAGATAAACTGTAATCAGGCATAA; GCTTGTTTGTATGTTCTGGCAGTGACTAATCAGTAAATGATGTCGGTTTGCCCAGTATCACTTATCTTC; TGTATTTTTCCTCTGTCGTGTAAATAGTATAACCTTTTCATTTATGGACAATTTTTTGGACTAGTAGCC; TTCAATATACATTCTGCTTTGAATTAATTTTTTCAAATCAATAAATTATGTAGACATTTAAAATCAAAT; ATCAAGTAGAATTGAAAAATGTGAGTTACATAAGTTAAAAACTTACTTTAAATCTTACCTTCTATAGGT; AGCTCTAAATAAATTCATATGGTTATATGGCATCTCTGGTGTATACTGATTGAGAAAATAATTAAACTG; AAGTTAGGGGAGGGGGA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000860.6
• Locus ID: 3248
• Summary: This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]