WNT3A (NM_033131) Human 3' UTR Clone

SKU SC216481

3' UTR clone of wingless-type MMTV integration site family member 3A (WNT3A) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: WNT3A
• ACCN: NM_033131
• Insert Size: 1836 bp
• Sequence Data:
  Insert Sequence

  >SC216481 3’UTR clone of NM_033131 ; The sequence shown below is from the reference sequence of NM_033131. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CGCGTCTACGACGTGCACACCTGCAAGTAGGCACCGGCCGCGGCTCCCCCTGGACGGGGCGGGCCCTGC; CTGAGGGTGGGCTTTTCCCTGGGTGGAGCAGGACTCCCACCTAAACGGGGCAGTACTCCTCCCTGGGGG; CGGGACTCCTCCCTGGGGGTGGGGCTCCTACCTGGGGGCAGAACTCCTACCTGAAGGCAGGGCTCCTCC; CTGGAGCTAGTGTCTCCTCTCTGGTGGCTGGGCTGCTCCTGAATGAGGCGGAGCTCCAGGATGGGGAGG; GGCTCTGCGTTGGCTTCTCCCTGGGGACGGGGCTCCCCTGGACAGAGGCGGGGCTACAGATTGGGCGGG; GCTTCTCTTGGGTGGGACAGGGCTTCTCCTGCGGGGGCGAGGCCCCTCCCAGTAAGGGCGTGGCTCTGG; GTGGGCGGGGCACTAGGTAGGCTTCTACCTGCAGGCGGGGCTCCTCCTGAAGGAGGCGGGGCTCTAGGA; TGGGGCACGGCTCTGGGGTAGGCTGCTCCCTGAGGGCGGAGCGCCTCCTTAGGAGTGGGGTTTTATGGT; GGATGAGGCTTCTTCCTGGATGGGGCAGAGCTTCTCCTGACCAGGGCAAGGCCCCTTCCACGGGGGCTG; TGGCTCTGGGTGGGCGTGGCCTGCATAGGCTCCTTCCTGTGGGTGGGGCTTCTCTGGGACCAGGCTCCA; ATGGGGCGGGGCTTCTCTCCGCGGGTGGGACTCTTCCCTGGGAACCGCCCTCCTGATTAAGGCGTGGCT; TCTGCAGGAATCCCGGCTCCAGAGCAGGAAATTCAGCCCACCAGCCACCTCATCCCCAACCCCCTGTAA; GGTTCCATCCACCCCTGCGTCGAGCTGGGAAGGTTCCATGAAGCGAGTCGGGTCCCCAACCCGTGCCCC; TGGGATCCGAGGGCCCCTCTCCAAGCGCCTGGCTTTGGAATGCTCCAGGCGCGCCGACGCCTGTGCCAC; CCCTTCCTCAGCCTGGGGTTTGACCACCCACCTGACCAGGGGCCCTACCTGGGGAAAGCCTGAAGGGCC; TCCCAGCCCCCAACCCCAAGACCAAGCTTAGTCCTGGGAGAGGACAGGGACTTCGCAGAGGCAAGCGAC; CGAGGCCCTCCCAAAGAGGCCCGCCCTGCCCGGGCTCCCACACCGTCAGGTACTCCTGCCAGGGAACTG; GCCTGCTGCGCCCCAGGCCCCGCCCGTCTCTGCTCTGCTCAGCTGCGCCCCCTTCTTTGCAGCTGCCCA; GCCCCTCCTCCCTGCCCTCGGGTCTCCCCACCTGCACTCCATCCAGCTACAGGAGAGATAGAAGCCTCT; CGTCCCGTCCCTCCCTTTCCTCCGCCTGTCCACAGCCCCTTAAGGGAAAGGTAGGAAGAGAGGTCCAGC; CCCCCAGGCTGCCCAGAGCTGCTGGTCTCATTTGGGGGCGTTCGGGAGGTTTGGGGGGCATCAACCCCC; CGACTGTGCTGCTCGCGAAGGTCCCACAGCCCTGAGATGGGCCGGCCCCCTTCCTGGCCCCTCATGGCG; GGACTGGAGAAATGGTCCGCTTTCCTGGAGCCAATGGCCCGGCCCCTCCTGACTCATCCGCCTGGCCCG; GGAATGAATGGGGAGGCCGCTGAACCCACCCGGCCCATATCCCTGGTTGCCTCATGGCCAGCGCCCCTC; AGCCTCTGCCACTGTGAACCGGCTCCCACCCTCAAGGTGCGGGGAGAAGAAGCGGCCAGGCGGGGCGCC; CCAAGAGCCCAAAAGAGGGCACACCGCCATCCTCTGCCTCAAATTCTGCGTTTTTGGTTTTAATGTTAT; ATCTGATGCTGCTATATCCACTGTCCAACGGAGTTAGACGAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033131.4
• Locus ID: 89780
• Summary: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]