HMGCR (NM_000859) Human 3' UTR Clone

CAT#: SC216360

3' UTR clone of 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGCR) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HMGCR
• Synonyms: LDLCQ3
• ACCN: NM_000859
• Insert Size: 1792 bp
• Sequence Data:
  Insert Sequence

  >SC216360 3' UTR clone of NM_000859
  The sequence shown below is from the reference sequence of NM_000859. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAGCCCGACAGTTCTGAACTGGAACATGG
  GCATTGGGTTCTAAAGGACTAACATAAAATCTGTGAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGA
  TGAATAGATGTGATCACTGAGACAGCCACTTGGTTTTTGGCTCTTTCAGAGAGGTCTCAGGTTCTTTCCA
  TGCAGACTCCTCAGATCTGAACACAGTTTAGTGCTTTACATGCTGTGCTCTTTGAAGAGATTTCAACAAG
  AATATTGTATGTTAAAGCATCAGAGATGGTAATCTACAGCTCACCTCTGAAGGCAAATATAAGCTGGGAA
  AAAAGTTTTGATGAAATTCTTGAAGTTCATGGTGATCAGTGCAATTGACCTTCTCCCTCACTCCTGCCAG
  TTGAAAATGGATTTTTAAATTATACTGTAGCTGATGAAACTCCTGATTTTGTAGTTAATTTATTAAGTCT
  GGGATGTAGAACTTCAAGAAGTAAGAGCTAAGTTCTAAGTTCATGTTTGTAAATTAATACTTCATTTGGT
  GCTGGTCTATTTTGATTTTGGGGGGTAATCAGCATTATTCTTCAGAAGGGGACCTGTTTTCTTCAAGGGA
  AGAAACACTCTTATTCCCAAACTACAGAATAATGTGTTAAACATGCTAAATAGTTCTATCAGGAAAACAA
  ATCACTGTATTTATCTCCGCAGGCTATTTGTTCAGAGAGGCCTTTTGTTTAAATATAAATGTTTAAATAT
  AAATGTTTGTCTGGATTGGCTATAACATGTCTTTCAGCATTAGGCTTTTAAGAAACACAGGGTTTTGTAT
  TCTTTACTAAAGATATCAGAGCTCTTAATGTTGCTTAGATGAGGGTGACTGTCAAGTACAAGCAAGACTG
  GGACCTTAGAAATCATTGTAGAAACACAGTTTTGAAAGAAAAATACCATGTCTCTAAGCCAACTTTAATT
  GCTTAAAAGACATTTTTATTTAGTTGAAAAATCTAGTTTTTTTTGTAAACTGTATCAAATCTGTATATGT
  TGTAATAAAACTTATGCTAGTTTATTGGAAGTGTTCAAGAAATAAAAATCAACTTGTGTACTGATAAAAT
  ACTCTAGCCTGGGCCAGAGAAGATAATGTTCTTTAATGTTGTCCAGGAAACCCTGGCTTGCTTGCCGAGC
  CTAATGAAAGGGAAAGTCAGCTTTCAGAGCCAGTGAAGGAGCCACGTGAATGGCCCTAGAACTGTGCCTA
  GTTCCTGTGGCCAGGAGGTTGGTGACTGAAACATTCACACAGGGCTCTTTGATGGACCCACGAACGCTCT
  TAGCTTTCTCAGGGGGTCAGCAGAGTTATTGAATCTTAATTTTTTTTAATGTACAAGTTTTGTATAAATA
  ATAAAGAACTCCTTATTTTGTATTACATCTAATGCTTCAAGTGTTGCTCTTGGAAAGCTGATGATGTCTC
  TTGTAGAAGATGGACTCTGAAAAACATTCCAGGAAACCATGGCAGCATGGAGAGCCTCTTAGTGATTGTG
  TCTGCATTGTTATTGTGGAAGATTTACCTTTTCTGTTGTACGTAAAGCTTAAATTGCTTTTGTTGTGACT
  TTTTAGCCAGTGACTTTTTCTGAGCTTTTCATGGAAGTGGCAGTGAAAAATATGTTGAGTGTTCATTTTA
  GTGACTGTAATTAATATCTTGCTGGATTAATGTTTTGTACAATTACTAAATTGTATACATTTTGTTATAG
  AATACTTTTTTCTAGTTTCAGTAAATAATGAAAAGGAAGTTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000859.2
• Summary: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Locus ID: 3156