HMGCR (NM_000859) Human 3' UTR Clone

SKU SC216360

3' UTR clone of 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGCR) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: HMGCR
• Synonyms: LDLCQ3
• ACCN: NM_000859
• Insert Size: 1792 bp
• Sequence Data:
  Insert Sequence

  >SC216360 3' UTR clone of NM_000859; The sequence shown below is from the reference sequence of NM_000859. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon ; ; ; CAATTGGCAGAGCTCAGAATTCAAGCGATCGC; ; ACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAGCCCGACAGTTCTGAACTGGAACATGG; GCATTGGGTTCTAAAGGACTAACATAAAATCTGTGAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGA; TGAATAGATGTGATCACTGAGACAGCCACTTGGTTTTTGGCTCTTTCAGAGAGGTCTCAGGTTCTTTCCA; TGCAGACTCCTCAGATCTGAACACAGTTTAGTGCTTTACATGCTGTGCTCTTTGAAGAGATTTCAACAAG; AATATTGTATGTTAAAGCATCAGAGATGGTAATCTACAGCTCACCTCTGAAGGCAAATATAAGCTGGGAA; AAAAGTTTTGATGAAATTCTTGAAGTTCATGGTGATCAGTGCAATTGACCTTCTCCCTCACTCCTGCCAG; TTGAAAATGGATTTTTAAATTATACTGTAGCTGATGAAACTCCTGATTTTGTAGTTAATTTATTAAGTCT; GGGATGTAGAACTTCAAGAAGTAAGAGCTAAGTTCTAAGTTCATGTTTGTAAATTAATACTTCATTTGGT; GCTGGTCTATTTTGATTTTGGGGGGTAATCAGCATTATTCTTCAGAAGGGGACCTGTTTTCTTCAAGGGA; AGAAACACTCTTATTCCCAAACTACAGAATAATGTGTTAAACATGCTAAATAGTTCTATCAGGAAAACAA; ATCACTGTATTTATCTCCGCAGGCTATTTGTTCAGAGAGGCCTTTTGTTTAAATATAAATGTTTAAATAT; AAATGTTTGTCTGGATTGGCTATAACATGTCTTTCAGCATTAGGCTTTTAAGAAACACAGGGTTTTGTAT; TCTTTACTAAAGATATCAGAGCTCTTAATGTTGCTTAGATGAGGGTGACTGTCAAGTACAAGCAAGACTG; GGACCTTAGAAATCATTGTAGAAACACAGTTTTGAAAGAAAAATACCATGTCTCTAAGCCAACTTTAATT; GCTTAAAAGACATTTTTATTTAGTTGAAAAATCTAGTTTTTTTTGTAAACTGTATCAAATCTGTATATGT; TGTAATAAAACTTATGCTAGTTTATTGGAAGTGTTCAAGAAATAAAAATCAACTTGTGTACTGATAAAAT; ACTCTAGCCTGGGCCAGAGAAGATAATGTTCTTTAATGTTGTCCAGGAAACCCTGGCTTGCTTGCCGAGC; CTAATGAAAGGGAAAGTCAGCTTTCAGAGCCAGTGAAGGAGCCACGTGAATGGCCCTAGAACTGTGCCTA; GTTCCTGTGGCCAGGAGGTTGGTGACTGAAACATTCACACAGGGCTCTTTGATGGACCCACGAACGCTCT; TAGCTTTCTCAGGGGGTCAGCAGAGTTATTGAATCTTAATTTTTTTTAATGTACAAGTTTTGTATAAATA; ATAAAGAACTCCTTATTTTGTATTACATCTAATGCTTCAAGTGTTGCTCTTGGAAAGCTGATGATGTCTC; TTGTAGAAGATGGACTCTGAAAAACATTCCAGGAAACCATGGCAGCATGGAGAGCCTCTTAGTGATTGTG; TCTGCATTGTTATTGTGGAAGATTTACCTTTTCTGTTGTACGTAAAGCTTAAATTGCTTTTGTTGTGACT; TTTTAGCCAGTGACTTTTTCTGAGCTTTTCATGGAAGTGGCAGTGAAAAATATGTTGAGTGTTCATTTTA; GTGACTGTAATTAATATCTTGCTGGATTAATGTTTTGTACAATTACTAAATTGTATACATTTTGTTATAG; AATACTTTTTTCTAGTTTCAGTAAATAATGAAAAGGAAGTTA; ; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000859.2
• Locus ID: 3156
• Summary: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]