GJA1 (NM_000165) Human 3' UTR Clone

SKU SC216210

3' UTR clone of gap junction protein alpha 1 43kDa (GJA1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: GJA1
• Synonyms: AVSD3; CMDR; CX43; EKVP; EKVP3; GJAL; HLHS1; HSS; ODDD; PPKCA
• ACCN: NM_000165
• Insert Size: 1761 bp
• Sequence Data:
  Insert Sequence

  >SC216210 3’UTR clone of NM_000165 ; The sequence shown below is from the reference sequence of NM_000165. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AGACCTCGGCCTGATGACCTGGAGATCTAGATACAGGCTTGAAAGCATCAAGATTCCACTCAATTGTGG; AGAAGAAAAAAGGTGCTGTAGAAAGTGCACCAGGTGTTAATTTTGATCCGGTGGAGGTGGTACTCAACA; GCCTTATTCATGAGGCTTAGAAAACACAAAGACATTAGAATACCTAGGTTCACTGGGGGTGTATGGGGT; AGATGGGTGGAGAGGGAGGGGATAAGAGAGGTGCATGTTGGTATTTAAAGTAGTGGATTCAAAGAACTT; AGATTATAAATAAGAGTTCCATTAGGTGATACATAGATAAGGGCTTTTTCTCCCCGCAAACACCCCTAA; GAATGGTTCTGTGTATGTGAATGAGCGGGTGGTAATTGTGGCTAAATATTTTTGTTTTACCAAGAAACT; GAAATAATTCTGGCCAGGAATAAATACTTCCTGAACATCTTAGGTCTTTTCAACAAGAAAAAGACAGAG; GATTGTCCTTAAGTCCCTGCTAAAACATTCCATTGTTAAAATTTGCACTTTGAAGGTAAGCTTTCTAGG; CCTGACCCTCCAGGTGTCAATGGACTTGTGCTACTATATTTTTTTATTCTTGGTATCAGTTTAAAATTC; AGACAAGGCCCACAGAATAAGATTTTCCATGCATTTGCAAATACGTATATTCTTTTTCCATCCACTTGC; ACAATATCATTACCATCACTTTTTCATCATTCCTCAGCTACTACTCACATTCATTTAATGGTTTCTGTA; AACATTTTTAAGACAGTTGGGATGTCACTTAACATTTTTTTTTTGAGCTAAAGTCAGGGAATCAAGCCA; TGCTTAATATTTAACAATCACTTATATGTGTGTCGAAGAGTTTGTTTTGTTTGTCATGTATTGGTACAA; GCAGATACAGTATAAACTCACAAACACAGATTTGAAAATAATGCACATATGGTGTTCAAATTTGAACCT; TTCTCATGGATTTTTGTGGTGTGGGCCAATATGGTGTTTACATTATATAATTCCTGCTGTGGCAAGTAA; AGCACACTTTTTTTTTCTCCTAAAATGTTTTTCCCTGTGTATCCTATTATGGATACTGGTTTTGTTAAT; TATGATTCTTTATTTTCTCTCCTTTTTTTAGGATATAGCAGTAATGCTATTACTGAAATGAATTTCCTT; TTTCTGAAATGTAATCATTGATGCTTGAATGATAGAATTTTAGTACTGTAAACAGGCTTTAGTCATTAA; TGTGAGAGACTTAGAAAAAATGCTTAGAGTGGACTATTAAATGTGCCTAAATGAATTTTGCAGTAACTG; GTATTCTTGGGTTTTCCTACTTAATACACAGTAATTCAGAACTTGTATTCTATTATGAGTTTAGCAGTC; TTTTGGAGTGACCAGCAACTTTGATGTTTGCACTAAGATTTTATTTGGAATGCAAGAGAGGTTGAAAGA; GGATTCAGTAGTACACATACAACTAATTTATTTGAACTATATGTTGAAGACATCTACCAGTTTCTCCAA; ATGCCTTTTTTAAAACTCATCACAGAAGATTGGTGAAAATGCTGAGTATGACACTTTTCTTCTTGCATG; CATGTCAGCTACATAAACAGTTTTGTACAATGAAAATTACTAATTTGTTTGACATTCCATGTTAAACTA; CGGTCATGTTCAGCTTCATTGCATGTAATGTAGACCTAGTCCATCAGATCATGTGTTCTGGAGAGTGTT; CTTTATTCAATAAAGTTTTAATTTAGTATAAACATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000165.5
• Locus ID: 2697
• Summary: This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]