SOCS3 (NM_003955) Human 3' UTR Clone

CAT#: SC215826

3' UTR clone of suppressor of cytokine signaling 3 (SOCS3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SOCS3
• Synonyms: ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3
• ACCN: NM_003955
• Insert Size: 1670 bp
• Sequence Data:
  Insert Sequence

  >SC215826 3’UTR clone of NM_003955
  The sequence shown below is from the reference sequence of NM_003955. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTCCTGGACCAGTACGATGCCCCGCTTTAAGGGGTAAAGGGCGCAAAGGGCATGGGTCGGGAGAGGGGA
  CGCAGGCCCCTCTCCTCCGTGGCACATGGCACAAGCACAAGAAGCCAACCAGGAGAGAGTCCTGTAGCT
  CTGGGGGGAAAGAGGGCGGACAGGCCCCTCCCTCTGCCCTCTCCCTGCAGAATGTGGCAGGCGGACCTG
  GAATGTGTTGGAGGGAAGGGGGAGTACCACCTGAGTCTCCAGCTTCTCCGGAGGAGCCAGCTGTCCTGG
  TGGGACGATAGCAACCACAAGTGGATTCTCCTTCAATTCCTCAGCTTCCCCTCTGCCTCCAAACAGGGG
  ACACTTCGGGAATGCTGAACTAATGAGAACTGCCAGGGAATCTTCAAACTTTCCAACGGAACTTGTTTG
  CTCTTTGATTTGGTTTAAACCTGAGCTGGTTGTGGAGCCTGGGAAAGGTGGAAGAGAGAGAGGTCCTGA
  GGGCCCCAGGGCTGCGGGCTGGCGAAGGAAATGGTCACACCCCCCGCCCACCCCAGGCGAGGATCCTGG
  TGACATGCTCCTCTCCCTGGCTCCGGGGAGAAGGGCTTGGGGTGACCTGAAGGGAACCATCCTGGTACC
  CCACATCCTCTCCTCCGGGACAGTCACCGAAAACACAGGTTCCAAAGTCTACCTGGTGCCTGAGAGCCC
  AGGGCCCTTCCTCCGTTTTAAGGGGGAAGCAACATTTGGAGGGGATGGATGGGCTGGTCAGCTGGTCTC
  CTTTTCCTACTCATACTATACCTTCCTGTACCTGGGTGGATGGAGCGGGAGGATGGAGGAGACGGGACA
  TCTTTCACCTCAGGCTCCTGGTAGAGAAGACAGGGGATTCTACTCTGTGCCTCCTGACTATGTCTGGCT
  AAGAGATTCGCCTTAAATGCTCCCTGTCCCATGGAGAGGGACCCAGCATAGGAAAGCCACATACTCAGC
  CTGGATGGGTGGAGAGGCTGAGGGACTCACTGGAGGGCACCAAGCCAGCCCACAGCCAGGGAAGTGGGG
  AGGGGGGGCGGAAACCCATGCCTCCCAGCTGAGCACTGGGAATGTCAGCCCAGTAAGTATTGGCCAGTC
  AGGCGCCTCGTGGTCAGAGCAGAGCCACCAGGTCCCACTGCCCCGAGCCCTGCACAGCCCTCCCTCCTG
  CCTGGGTGGGGGAGGCTGGAGGTCATTGGAGAGGCTGGACTGCTGCCACCCCGGGTGCTCCCGCTCTGC
  CATAGCACTGATCAGTGACAATTTACAGGAATGTAGCAGCGATGGAATTACCTGGAACAGTTTTTTGTT
  TTTGTTTTTGTTTTTGTTTTTGTGGGGGGGGGCAACTAAACAAACACAAAGTATTCTGTGTCAGGTATT
  GGGCTGGACAGGGCAGTTGTGTGTTGGGGTGGTTTTTTTCTCTATTTTTTTGTTTGTTTCTTGTTTTTT
  AATAATGTTTACAATCTGCCTCAATCACTCTGTCTTTTATAAAGATTCCACCTCCAGTCCTCTCTCCTC
  CCCCCTACTCAGGCCCTTGAGGCTATTAGGAGATGCTTGAAGAACTCAACAAAATCCCAATCCAAGTCA
  AACTTTGCACATATTTATATTTATATTCAGAAAAGAAACATTTCAGTAATTTATAATAAAGAGCACTAT
  TTTTTAATGAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003955.5
• Summary: This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]
• Locus ID: 9021
• MW: 62.1