SOCS3 (NM_003955) Human 3' UTR Clone

SKU SC215826

3' UTR clone of suppressor of cytokine signaling 3 (SOCS3) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: SOCS3
• Synonyms: ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3
• ACCN: NM_003955
• Insert Size: 1670 bp
• Sequence Data:
  Insert Sequence

  >SC215826 3’UTR clone of NM_003955 ; The sequence shown below is from the reference sequence of NM_003955. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TTCCTGGACCAGTACGATGCCCCGCTTTAAGGGGTAAAGGGCGCAAAGGGCATGGGTCGGGAGAGGGGA; CGCAGGCCCCTCTCCTCCGTGGCACATGGCACAAGCACAAGAAGCCAACCAGGAGAGAGTCCTGTAGCT; CTGGGGGGAAAGAGGGCGGACAGGCCCCTCCCTCTGCCCTCTCCCTGCAGAATGTGGCAGGCGGACCTG; GAATGTGTTGGAGGGAAGGGGGAGTACCACCTGAGTCTCCAGCTTCTCCGGAGGAGCCAGCTGTCCTGG; TGGGACGATAGCAACCACAAGTGGATTCTCCTTCAATTCCTCAGCTTCCCCTCTGCCTCCAAACAGGGG; ACACTTCGGGAATGCTGAACTAATGAGAACTGCCAGGGAATCTTCAAACTTTCCAACGGAACTTGTTTG; CTCTTTGATTTGGTTTAAACCTGAGCTGGTTGTGGAGCCTGGGAAAGGTGGAAGAGAGAGAGGTCCTGA; GGGCCCCAGGGCTGCGGGCTGGCGAAGGAAATGGTCACACCCCCCGCCCACCCCAGGCGAGGATCCTGG; TGACATGCTCCTCTCCCTGGCTCCGGGGAGAAGGGCTTGGGGTGACCTGAAGGGAACCATCCTGGTACC; CCACATCCTCTCCTCCGGGACAGTCACCGAAAACACAGGTTCCAAAGTCTACCTGGTGCCTGAGAGCCC; AGGGCCCTTCCTCCGTTTTAAGGGGGAAGCAACATTTGGAGGGGATGGATGGGCTGGTCAGCTGGTCTC; CTTTTCCTACTCATACTATACCTTCCTGTACCTGGGTGGATGGAGCGGGAGGATGGAGGAGACGGGACA; TCTTTCACCTCAGGCTCCTGGTAGAGAAGACAGGGGATTCTACTCTGTGCCTCCTGACTATGTCTGGCT; AAGAGATTCGCCTTAAATGCTCCCTGTCCCATGGAGAGGGACCCAGCATAGGAAAGCCACATACTCAGC; CTGGATGGGTGGAGAGGCTGAGGGACTCACTGGAGGGCACCAAGCCAGCCCACAGCCAGGGAAGTGGGG; AGGGGGGGCGGAAACCCATGCCTCCCAGCTGAGCACTGGGAATGTCAGCCCAGTAAGTATTGGCCAGTC; AGGCGCCTCGTGGTCAGAGCAGAGCCACCAGGTCCCACTGCCCCGAGCCCTGCACAGCCCTCCCTCCTG; CCTGGGTGGGGGAGGCTGGAGGTCATTGGAGAGGCTGGACTGCTGCCACCCCGGGTGCTCCCGCTCTGC; CATAGCACTGATCAGTGACAATTTACAGGAATGTAGCAGCGATGGAATTACCTGGAACAGTTTTTTGTT; TTTGTTTTTGTTTTTGTTTTTGTGGGGGGGGGCAACTAAACAAACACAAAGTATTCTGTGTCAGGTATT; GGGCTGGACAGGGCAGTTGTGTGTTGGGGTGGTTTTTTTCTCTATTTTTTTGTTTGTTTCTTGTTTTTT; AATAATGTTTACAATCTGCCTCAATCACTCTGTCTTTTATAAAGATTCCACCTCCAGTCCTCTCTCCTC; CCCCCTACTCAGGCCCTTGAGGCTATTAGGAGATGCTTGAAGAACTCAACAAAATCCCAATCCAAGTCA; AACTTTGCACATATTTATATTTATATTCAGAAAAGAAACATTTCAGTAATTTATAATAAAGAGCACTAT; TTTTTAATGAAAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003955.5
• Locus ID: 9021
• Summary: This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]