ROCK1 (NM_005406) Human 3' UTR Clone

SKU SC215786

3' UTR clone of Rho-associated coiled-coil containing protein kinase 1 (ROCK1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: ROCK1
• Synonyms: P160ROCK; ROCK-I
• ACCN: NM_005406
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC215786 3’UTR clone of NM_005406 ; The sequence shown below is from the reference sequence of NM_005406. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GTCAAAAATACATCTGGAAAAACTAGTTAACCATGTGACTGAGTGCCCTGTGGAATCGTGTGGGATGCT; ACCTGATAAACCAGGCTTCTTTAACCATGCAGAGCAGACAGGCTGTTTCTTTGACACAAATATCACAGG; CTTCAGGGTTAAGATTGCTGTTTTTCTGTCCTTGCTTTGGCACAACACACTGAGGGTTTTTTTTATTGC; GGGTTTGCCTACAGGTAGATTAGATTAATTATTACTATGTAATGCAAGTACAGTTGGGGGAAAGCTTAG; GTAGATATATTTTTTTTAAAAGGTGCTGCCTTTTTGGATTTATAAGAAAATGCCTGTCAGTCGTGATAG; AACAGAGTTTTCCTCATATGAGTAAGAGGAAGGGACTTTCACTTTCAAGTGGAACAGCCATCACTATCA; AGATCAGCTCATGGAAGGAGTAAAGAAAATATCTCAAAATGAGACAAACTGAAGTTTTGTTTTTTTTTT; AATGACTTAAGTTTTTGTGCTCTTGCAAGACTATACAAAACTATTTTAAGAAAGCAGTGATATCACTTG; AACTTCAGTGCCCTCACTGTAGAATTTAAAAGCCTTACTGTTGATTGCCCATGTTGGACTTGATGGAGA; AATTAAATATCTTTCATTATGCTTTACAAAATACTGTATATGTTTCAGCAAGTTTGGGGAATGGGAGAG; GACAAAAAAAAGTTACATTTAATCTATGCATTTTTGCCAAGCCATATTGAGTTATTTTACTACTAGAGA; CATTAGGAAACTAACTGTACAAAAGAACCAAGTTTAAAAGCATTTTGTGGGGTACATCATTTCTATAAT; TGTATAATGTATTTCTTTGTGGTTTTAAATGATAAAGACATTAAGTTAACAAACATATAAGAAATGTAT; GCACTGTTTGAAATGTAAATTATTCTTAGAACACTTTCAATGGGGGTTGCATTGTCCTTTTAGTGCCTT; AATTTGAGATAATTATTTTACTGCCATGAGTAAGTATAGAAATTTCAAAAAATGTATTTTCAAAAAATT; ATGTGTGTCAGTGAGTTTTTCATTGATAATTGGTTTAATTTAAAATATTTAGAGGTTTGTTGGACTTTC; ATAAATTGAGTACAATCTTTGCATCAAACTACCTGCTACAATAATGACTTTATAAAACTGCAAAAAATG; TAGAAGGTTGCACCAACATAAAAAGGAAATATGGCAATACATCCATGATGTTTTCCAGTTAACATAGGA; ATTACCAGATAAATACTGTTAAACTCTTGTCCAGTAACAAGAGTTGATTCATATGGACAGTATGATTTA; TTGTTTATTTTTTTAACCAAATACCTCCTCAGTAATTTATAATGGCTTTGCAGTAATGTGTATCAGATA; AGAAGCACTGGAAAACCGATCGTCTCTAGGATGATATGCATGTTTCAAGTGGTATTGAAAGCCGCACTG; ATGGATATGTAATAATAAACATATCTGTTATTAATATACTAATGACTCTGTGCTCATTTAATGAGAAAT; AAAAGTAATTTATGGATGGGTATCTTTAATTTTTACTGCAATGTGTTTTCTCATGGCTGAAATGAATGG; AAAACATACTTCAAATTAGTCTCTGATTGTATATAAATGTTTGTGAAATTCCATGGTTAGATTAAAGTG; TATTTTTAAAAGATAAAACGTGGTCTTGGGCTCTAAAACAAGAGTGGGAATAGTTCACATGAAAAGCTG; GCTAAAGTGTGTTGGAAAACAATTCTCCATGGGTCTCTTGCATTTCTACACATCAGGGAAGCAGAGGCT; CTGACAACCTTTCTTTTGACTATCTTTTCCAAGGATATTTATGTAGTAAACAGCCTTGGAAGATAGAGT; TCTCCCTTGTGAGGATGTCGGGTTCTTTTACTGTCCAGTATAATAAAGATAACGTCTCCCACCTATGAT; GCAAACCCACTGTGTGCTCAGCACCCACCTGGGCCACTCTGCATTGCCCACATGGGGCTTGGAGGGCA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005406.3
• Locus ID: 6093
• Summary: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]