TMEM16A (ANO1) (NM_018043) Human 3' UTR Clone

CAT#: SC215210

3' UTR clone of anoctamin 1 calcium activated chloride channel (ANO1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TMEM16A
• Synonyms: DOG1; ORAOV2; TAOS2; TMEM16A
• ACCN: NM_018043
• Insert Size: 1556 bp
• Sequence Data:
  Insert Sequence

  >SC215210 3’UTR clone of NM_018043
  The sequence shown below is from the reference sequence of NM_018043. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGCCATGCCTACCACGGGGGCGTCCTGTAGCTATGCCAGCGGGGCTGGGCAGGCCAGCCGGGCATCCTG
  ACCGATGGGCACCCTCTCCCAGGGCAGGCGGCTTCCCGCTCCCACCAGGGCCCGGTGGGTCCTGGGTTT
  TCTGCAAACATGGAGGACCACTTTCTGATAGGACATTTTCCTTTCTTCTTTCTGTTTTCTTTCCCTTGT
  TTTTGCACAAAGCCATTATGCAGGGAATATTTTTTAATCTGTAGTATTCAAGATGAATCAAAATGATGG
  CTGGTAATACGGCAATAAGGTAGCAAAGGCAGGTGCTTTGCAGAAAGAATGCTTGGAAACTTGAGTCTC
  CCTAGAGGTGAAAAGTGAGCAGAGGCCCGTAGAAACCCTCCTCTGAATCCTCCTAATTCCTTAAGATAG
  ATGCAAAATGGTAAGCCGAGGCATCGCGCAAAAGCTGGTGCGATGCTTCAGGGAAAATGGAAAACCCAC
  GCAAGAATAATGATTGATTCCGGTTCCAAAAGGTGTCACCTACCTGTTTCAGAAAAGTTAGACTTTCCA
  TCGCCTTTTCCTTCCATCAGTTGAGTGGCTGAGAGAGAAGTGCCTCATCCCTGAGCCACACAGGGGGCG
  TGGGAGCATCCCAGTTATCCCTGGAAAGCTAGAAGGGGACAGAGGTGTCCCTGATTAAGCAGGAAACAG
  CACCCTTGGCGTCCCCAGCAGGCTCCCCACTGTCAGCCACACACCTGCCCCCATCACACCAAGCCGACC
  TCAGAGTTGTTCATCTTCCTTATGGGACAAAACCGGTTGACCAGAAAATGGGCAGAGAGAGATGACCTC
  GGAAGCATTTCCACAGATGGTGTCAGGGTTTCAAGAAGTCTTAGGGCTTCCAGGGGTCCCCTGGAAGCT
  TTAGAATATTTATGGGTTTTTTTTCAAATATCAATTATATGGTAGATTGAGGATTTTTTTTCTGTAGCT
  CAAAGGTGGAGGGAGTTTATTAGTTAACCAAATATCGTTGAGAGGAATTTAAAATACTGTTACTACCAA
  AGATTTTTATTAATAAAGGCTTATATTTTGGTAACACTTCTCTATATTTTTACTCACAGGAATGTCACT
  GTTGGACAATTATTTTAAAAGTGTATAAAACCAAGTCTCATAAATGATATGAGTGATCTAAATTTGCAG
  CAATGATACTAAACAACTCTCTGAAATTTCTCAAGCACCAAGAGAAACATCATTTTAGCAAAGGCCAGG
  AGGAAAAATAGAAATAAATTTGTCTTGAAGATCTCATTGATGTGATGTTACATTCCCTTTAATCTGCCA
  ACTGTGGTCAAAGTTCATAGGTGTCGTACATTTCCATTATTTGCTAAAATCATGCAATCTGATGCTTCT
  CTTTTCTCTTGTACAGTAAGTAGTTTGAAGTGGGTTTTGTATATAAATACTGTATTAAAAATTAGGCAA
  TTACCAAAAATCCTTTTATGGAAACCATTTTTTTAAAAAGTGAATGTACACAAATCCACAGAGGACTGT
  GGCTGGACATTCATCTAAATAAATTTGAATATACGACA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_018043.7
• Summary: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function]
• Locus ID: 55107
• MW: 59.9