MADH7 (SMAD7) (NM_005904) Human 3' UTR Clone

SKU SC215141

3' UTR clone of SMAD family member 7 (SMAD7) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: MADH7
• Synonyms: CRCS3; MADH7; MADH8
• ACCN: NM_005904
• Insert Size: 1549 bp
• Sequence Data:
  Insert Sequence

  >SC215141 3’UTR clone of NM_005904 ; The sequence shown below is from the reference sequence of NM_005904. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TGGCTAGAGGTCATCTTCAACAGCCGGTAGCCGCGTGCGGAGGGGACAGAGCGTGAGCTGAGCAGGCCA; CACTTCAAACTACTTTGCTGCTAATATTTTCCTCCTGAGTGCTTGCTTTTCATGCAAACTCTTTGGTCG; TTTTTTTTTTGTTTGTTGGTTGGTTTTCTTCTTCTCGTCCTCGTTTGTGTTCTGTTTTGTTTCGCTCTT; TGAGAAATAGCTTATGAAAAGAATTGTTGGGGGTTTTTTTGGAAGAAGGGGCAGGTATGATCGGCAGGA; CACCCTGATAGGAAGAGGGGAAGCAGAAATCCAAGCACCACCAAACACAGTGTATGAAGGGGGGCGGTC; ATCATTTCACTTGTCAGGAGTGTGTGTGAGTGTGAGTGTGCGGCTGTGTGTGCACGCGTGTGCAGGAGC; GGCAGATGGGGAGACAACGTGCTCTTTGTTTTGTGTCTCTTATGGATGTCCCCAGCAGAGAGGTTTGCA; GTCCCAAGCGGTGTCTCTCCTGCCCCTTGGACACGCTCAGTGGGGCAGAGGCAGTACCTGGGCAAGCTG; GCGGCTGGGGTCCCAGCAGCTGCCAGGAGCACGGCTCTGTCCCCAGCCTGGGAAAGCCCCTGCCCCTCC; TCTCCCTCATCAAGGACACGGGCCTGTCCACAGGCTTCTGAGCAGCGAGCCTGCTAGTGGCCGAACCAG; AACCAATTATTTTCATCCTTGTCTTATTCCCTTCCTGCCAGCCCCTGCCATTGTAGCGTCTTTCTTTTT; TGGCCATCTGCTCCTGGATCTCCCTGAGATGGGCTTCCCAAGGGCTGCCGGGGCAGCCCCCTCACAGTA; TTGCTCACCCAGTGCCCTCTCCCCTCAGCCTCTCCCCTGCCTGCCCTGGTGACATCAGGTTTTTCCCGG; ACTTAGAAAACCAGCTCAGCACTGCCTGCTCCCATCCTGTGTGTTAAGCTCTGCTATTAGGCCAGCAAG; CGGGGATGTCCCTGGGAGGGACATGCTTAGCAGTCCCCTTCCCTCCAAGAAGGATTTGGTCCGTCATAA; CCCAAGGTACCATCCTAGGCTGACACCTAACTCTTCTTTCATTTCTTCTACAACTCATACACTCGTATG; ATACTTCGACACTGTTCTTAGCTCAATGAGCATGTTTAGACTTTAACATAAGCTATTTTTCTAACTACA; AAGGTTTAAATGAACAAGAGAAGCATTCTCATTGGAAATTTAGCATTGTAGTGCTTTGAGAGAGAAAGG; ACTCCTGAAAAAAAACCTGAGATTTATTAAAGAAAAAAATGTATTTTATGTTATATATAAATATATTAT; TACTTGTAAATATAAAGACGTTTTATAAGCATCATTATTTATGTATTGTGCAATGTGTATAAACAAGAA; AAATAAAGAAAAGATGCACTTTGCTTTAATATAAATGCAAATAACAAATGCCAAATTAAAAAAGATAAA; CACAAGATTGGTGTTTTTTTCTATGGGTGTTATCACCTAGCTGAATGTTTTTCTAAAGGAGTTTATGTT; CCATTAAACGATTTTTAAAATGTACACTTGA; AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC; CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC;
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005904.4
• Locus ID: 4092
• Summary: The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]