GLUT4 (SLC2A4) (NM_001042) Human 3' UTR Clone

SKU SC214541

3' UTR clone of solute carrier family 2 (facilitated glucose transporter) member 4 (SLC2A4) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: GLUT4
• Synonyms: GLUT4
• ACCN: NM_001042
• Insert Size: 1658 bp
• Sequence Data:
  Insert Sequence

  >SC214541 3’UTR clone of NM_001042 ; The sequence shown below is from the reference sequence of NM_001042. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GAGTATTTAGGGCCAGATGAGAACGACTGAGGGGCCAGGCAGGGGTGGGAGAGCCAGCTCTCTCTACCC; GGCCCAGAGACCCCTTCCTTTCCTCTGCAGCACTTTAACCCTCTCTTCCCTATTATTTCCGGGTGGAAA; AGAATCCCTGCAGCCTGGTAGAATTGGGAAGCTGGGGGAAGGGTGGTCTGAGCACCCCCTCATTCCCCT; CGTGTGACTCTCTTGGATTATTTATGTGTTGTGGTTTGGCCGTGGCCATCAGGGTGGGCCACTCTCCCC; TCCCTCTTCCTTCCCCCATCCCCTTTCCTCCCCACCTTCCCCAGACTCAGCTCCAGAATACCTTCTTCG; CTGCTAGAGAAGGGGGATTGGAGGGAAGACAGGTCTAGACTTTCTCAGTGGGACAAACCAGAGCAGAGA; GCAGGACAGGAGACAAGAAATCCAGTTTCCCACCACCTTGGACTCCTCCCACAATCTGGGACTTTCACT; GAATTCTTGCCACGCAGACTCTGGGCAAAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACAG; TCTCGCTCTGTCGCCCAGGCTCGAGTGCAGTGGCGTGATCTTGCTTCACTGCAAGCTGTCTCCCAGGTT; CACGCCATTCTCCTGCCTCAGCCTCCGGAGTAGCTGGGACTACAGGCGCATGCCACCACACCTGGCTAA; TTTATTTTGTATTTTTAGTATATACGCGGTTTCACCATGTTAGCCAGAATGGTCTCGATCTCCTGACCT; CGTGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCTGGCGAAGG; GAGTTCTCTCTTGACCCCTGCAGGGAAAAGGACTCACCTCCCTCACTGCAGGCTCAGCCTTCCAGGGCA; AGAGGGAACAGGAAAGTATGTGCCCATGTGTGGCAAGATGGAAGGACGGCAGGCTCCCGCCTCTAGGCT; TGGGGCTCTACCCCGATGGTTTCCCAAGGCTGCCAAGAAGGAGCCCTAACTTTCTTCCTCTCCCTTCCT; GGAAGGGTGCTGCATCCACAGGCTTTTGACCAACTAAGGCAAAGAGGGGATTTGAAAGGCTGCCTGGAA; ACACTGGGCTGGGAGGAGCCTTTGGATATTTTTATATACGTTTGAAAAGGGGATTGAGAGAAGAAACCA; AAGGTCGGTTGTACTAAATGTATATATATAGATACTTCTATAAAGTCACTGCTGAAGACAAGCATCCTA; TTGTGGAGGTACTTGAGGATGGGCTGAGACAGGGACCATAACTCTTCACCCCTCTTCCTCCCTCTGTCC; TGCCTCAGCTCAAGGCCTCAGAATCTTCTGGATGCCATTGCTCATGCCCCTACTCACATTTCTACTCGT; TGCTTTATTAATAGTAAATGCTCAATAAATTGTAGCTGCCAGTGCCGGGCATTGCTCTTGGCATTTGCA; GAATACTCACTCTGTGAGGGAGGTGTCAGCCCATGTCACAGATGGGCAGTGAAACCCATGATAGGGGCA; CTCTTCACCAGGGACACAGCTGGAGACATGGCAGCAAGGATGCCAGGCCCTGCCCACGGCCCCACATCC; CCTCCTCCCCACCTTCAACAGTGAGTTACTAGCGATTCTCCAAAACAGAAGAGATGGAGACCAAGACCA; CA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001042.3
• Locus ID: 6517
• Summary: This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]