BORIS (CTCFL) (NM_080618) Human 3' UTR Clone

SKU SC214474

3' UTR clone of CCCTC-binding factor (zinc finger protein)-like (CTCFL) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: BORIS
• Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
• ACCN: NM_080618
• Insert Size: 1414 bp
• Sequence Data:
  Insert Sequence

  >SC214474 3’UTR clone of NM_080618 ; The sequence shown below is from the reference sequence of NM_080618. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GAAATGCTCCTCAACACGATGGATAAGTGAGAGGGATTCGGGTTGCGTGTTCACTGCCCCCAATTCCTA; AAGCAAGTTAGAAGTTTTTAGCATTTAAGGTGTGAAATGCTCCTCAACACGATGGATAAGTGAGAGAGA; GTCAGGTTGCATGTTCACTGCCCCTAATTCCTAAAGCAAGTTAGAAATTTTTAGCATTTTCTTTGAAAC; AATTAAGTTCATGACAATGGATGACACAAGTTTGAGGTAGTGTCTAGAATTGTTCTCCTGTTTGTAGCT; GGATATTTCAAAGAAACATTGCAGGTATTTTATAAAAGTTTTAAACCTTGAATGAGAGGGTAACACCTC; AAACCTATGGATTCATTCACTTGATATTGGCAAGGTGGCCCACAATGAGTGAGTAGTGATTTTTGGATA; TTTCAAAATAGTCTAGACCAGCTAGTGCTTCCACAGTCAAAGCTGGACATTTTTATGTTGCATTATATA; CACCCATGATATTTCTAATAATATATGGTTTTAAACATTAAAGACAAATGTTTTTATACAAATGAATTT; TCTACAAAATTTAAAGCTACCATAATGCTTTTAATTAGTTCTAAATTCAACCAAAAAATGTTTTACTCT; TATAAAAAGGAAAACTGAGTAGGAAATGAAATACTAGATTAGACTAGAAAATAAGGAATAAATCGATTT; TACTTTGGTATAGGAGCAAGGTTCACCTTTAGATTTTTGTATTCTCTTTTAATTATGCTCCTTGGCAGG; TATGAAATTGCCCTGGTTACATTCCATTATTGCTTATTAGTATTTCACTCCATAACCCTTTTTTCTGCT; AAAACTACTCTTTTTATATTTGTAAAATAATTGGCAGAGTGAGAAGAAACATAAAATCAGATAAGGCAA; ATGTGTACCTGTAAGGAATTTGTACTTTTTCATAATGCCCAGTGATTAGTGAGTATTTCCCTTTTGCCA; GTTGACAAGATTTTTCCACCCTCGAGCAGCGTGAGAGATGCCTCTTTAACACTTGAAATTCATTTCTAT; CTGGATACAGAGGCAGATTTTTCTTCATTGCTTAGTTGAGCAGTTTGTTTTGCTGCCAACCTGTCTCCA; CCCCTGTATTTCAAGATCATTGATAAGCCCTAAATTCAAATTCTTAAGATATGGACCTTTTATTGAAAA; TATCACAAGTTCAGAATCCCTATACAATGTGAATATGTGGAAATAATTTCCCAGCAGGAAGAGCATTAT; ATTCTCTTTGTACCAGCAAATTAATTTAACTCAACTCACATGAGATTTAAATTCTGTGGGCTGTAGTAT; GCCATCATTGTGACTGAATTTGTGCAATGGTTTCTTAATTTTTTTACTGTTATTTAAAGATGTTTTACA; TAATTCAATAAAATGAAATGACTTAAAATTGCTA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_080618.4
• Locus ID: 140690
• Summary: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]