ATF1 (NM_005171) Human 3' UTR Clone

SKU SC214060

3' UTR clone of activating transcription factor 1 (ATF1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: ATF1
• Synonyms: EWS-ATF1; FUS/ATF-1; TREB36
• ACCN: NM_005171
• Insert Size: 1413 bp
• Sequence Data:
  Insert Sequence

  >SC214060 3’UTR clone of NM_005171 ; The sequence shown below is from the reference sequence of NM_005171. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AAGGATCTTTATTCCAATAAAAGTGTTTGATTCCTAAGAAAGAAAATATTTTTGTGGACATGCATAAAA; ATTAAATGGATTTCCTAGTGGAGTTTTATAAATTAAAAGGTCAAAACTGAAGCTTTTTATTTAGGCTTT; TCCAAATCAAGGATAAATATCTTACGCACGATATCTAGTGACAGAGGAGAAAGTGGAAAATGACCTCAA; GGAAGCTACGGGCACAACTGGAAGCTTTGTAGAAATTAAACATATTCAAGGAGCAAGAAATGAACTTTC; AGCAGTCTAAATTTTCTAAATAACCAATAGTTGCCAATCTAAAATGGCAGAGAAGATGAAATTTGATAA; ACTGAATTTTTTTTAAAAATCCATTTACCCTACAGGTTTGCATTTGTTTGCTGAAATTTACCTTTTTTT; AGTTATATATATGTGTGTGTGTGTGTGTAATTTCTGCCAATAAATTCTAAATTACAAAGGTAAGAGAAA; ACCTAGTACATTACTAAATATATAAAGTATATGTTCTGATTATGTATACTTGTTCTAGTGTCAAGTCTT; TTTAAGTGGGTTTTTAAAAGTTTGTTATTGGACTTGAATGGATTTTTGAGACTAGGTTAATTATTTTTG; AGGTCTTATCCTAAAAGGCATCTAAGGTACATGAATGGAGTATGGTGATTTTATAACATTTTTTATCAG; AATGGAAAAAGAACTGTTTAAAAGTTTGATACTTTTAAATAGTTGGTTTTTTTGCTTACTCTGGTAATG; ATTTTCTACAAATACATAATAAATTGTTTTTTTGAGTCTATATTCTGTATGCAGTTGAATATCCATTAC; TTATTCTGCTGTGCTTTAATAGAATGGAATGTTTACAGGCCCTTAAAATATTATTTTTAAAAAACCTTC; TGAAGATACATACCAAAGTTTTTCCAAGAAGATTTTATAATCAATTTAATAATGTAAGGTTTATCAGAT; TCTATAATAGTATAGTTATTAAGGCAATTTTATGTTAGAGACTATTTTGTAATGTAGTGAGTGGTACCT; TTATAAGAAAAGTGACTGCCAATATATTTTTATAGCTGATCTTTATAAATTCTAATGTTGAGTTTTTAA; TGATTATTTTAAATGTTTATATAGTTTAGTAAAATTTGCATCTCAAAGTATCATTTTTATATTATGGGA; CGTTTTCAGATTGGCTAATATTTGCATTGTAAATTTTGTATGCAGTTTATCTAAAATTCAAAAATACTG; TCAGTACACCAGCGTTTAACATCTATATTCCAATTTGTATACAGTTTAAAATTGTACTGCAAAACTATT; GTGTGCTCTTACACAGTATGCATCATATTGTTGTCTGTGAAATTAAAGGACATTTGATAGTCTACTGAA; TGTAAAATATAATGCTTGGTATATGAATGATAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005171.5
• Locus ID: 466
• Summary: This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]