p27 KIP 1 (CDKN1B) (NM_004064) Human 3' UTR Clone

CAT#: SC214055

3' UTR clone of cyclin-dependent kinase inhibitor 1B (p27 Kip1) (CDKN1B) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: p27 KIP 1
• Synonyms: CDKN4; KIP1; MEN1B; MEN4; P27KIP1
• ACCN: NM_004064
• Insert Size: 1372 bp
• Sequence Data:
  Insert Sequence

  >SC214055 3’UTR clone of NM_004064
  The sequence shown below is from the reference sequence of NM_004064. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGCCTGGCCTCAGAAGACGTCAAACGTAAACAGCTCGAATTAAGAATATGTTTCCTTGTTTATCAGAT
  ACATCACTGCTTGATGAAGCAAGGAAGATATACATGAAAATTTTAAAAATACATATCGCTGACTTCATG
  GAATGGACATCCTGTATAAGCACTGAAAAACAACAACACAATAACACTAAAATTTTAGGCACTCTTAAA
  TGATCTGCCTCTAAAAGCGTTGGATGTAGCATTATGCAATTAGGTTTTTCCTTATTTGCTTCATTGTAC
  TACCTGTGTATATAGTTTTTACCTTTTATGTAGCACATAAACTTTGGGGAAGGGAGGGCAGGGTGGGGC
  TGAGGAACTGACGTGGAGCGGGGTATGAAGAGCTTGCTTTGATTTACAGCAAGTAGATAAATATTTGAC
  TTGCATGAAGAGAAGCAATTTTGGGGAAGGGTTTGAATTGTTTTCTTTAAAGATGTAATGTCCCTTTCA
  GAGACAGCTGATACTTCATTTAAAAAAATCACAAAAATTTGAACACTGGCTAAAGATAATTGCTATTTA
  TTTTTACAAGAAGTTTATTCTCATTTGGGAGATCTGGTGATCTCCCAAGCTATCTAAAGTTTGTTAGAT
  AGCTGCATGTGGCTTTTTTAAAAAAGCAACAGAAACCTATCCTCACTGCCCTCCCCAGTCTCTCTTAAA
  GTTGGAATTTACCAGTTAATTACTCAGCAGAATGGTGATCACTCCAGGTAGTTTGGGGCAAAAATCCGA
  GGTGCTTGGGAGTTTTGAATGTTAAGAATTGACCATCTGCTTTTATTAAATTTGTTGACAAAATTTTCT
  CATTTTCTTTTCACTTCGGGCTGTGTAAACACAGTCAAAATAATTCTAAATCCCTCGATATTTTTAAAG
  ATCTGTAAGTAACTTCACATTAAAAAATGAAATATTTTTTAATTTAAAGCTTACTCTGTCCATTTATCC
  ACAGGAAAGTGTTATTTTTCAAGGAAGGTTCATGTAGAGAAAAGCACACTTGTAGGATAAGTGAAATGG
  ATACTACATCTTTAAACAGTATTTCATTGCCTGTGTATGGAAAAACCATTTGAAGTGTACCTGTGTACA
  TAACTCTGTAAAAACACTGAAAAATTATACTAACTTATTTATGTTAAAAGATTTTTTTTAATCTAGACA
  ATATACAAGCCAAAGTGGCATGTTTTGTGCATTTGTAAATGCTGTGTTGGGTAGAATAGGTTTTCCCCT
  CTTTTGTTAAATAATATGGCTATGCTTAAAAGGTTGCATACTGAGCCAAGTATAATTTTTTGTAATGTG
  TGAAAAAGATGCCAATTATTGTTACACATTAAGTAATCAATAAAGAAAACTTCCATAGCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004064.5
• Summary: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
• Locus ID: 1027
• MW: 52.8