CDC7 (NM_003503) Human 3' UTR Clone

SKU SC214002

3' UTR clone of cell division cycle 7 homolog (S. cerevisiae) (CDC7) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: CDC7
• Synonyms: CDC7L1; HsCDC7; Hsk1; huCDC7
• ACCN: NM_003503
• Insert Size: 1359 bp
• Sequence Data:
  Insert Sequence

  >SC214002 3’UTR clone of NM_003503 ; The sequence shown below is from the reference sequence of NM_003503. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CATCCATTTTTTAAAGATATGAGCTTGTGATAATGGATCTTCATTTAATGTTTACTGTTATGAGGTAGA; ATAAAAAAGAATACTTTGTAATAGCCACAAGTTCTTGTTTAGAGACCAGAGCAGGATTAATAATTTATT; TTAACATTTTAGTGTTTGGTGGCACATTCTAAAATATAGATTAAGAATACTTAAAATGCCTGGGATAGT; TCTTGGGACTAACAACATGATCTTCTTTGAGTTAAACCTACCTAAGTAGATTTTAGGTGGGTTCCTATT; AGGTCAGATTTTTAGCTTCCCTAATTACCTTTCACTGACATATACAGAAAAAGGAGCAGTTTTAGTTTT; AATTAATTAAAATTAACAGATGTGATGAGGATTAAATGAATCAAAAGACTTAATTTGTAGATTCTTTTA; GAGTTATGAGCTAGGTATAGTTTGGGGAAACTCAACCTGGTGCTGGTGCTCTTAACAATTTTGTAAATA; AAGAAGATAATTTCCTTTTCTAGAGGTACATATTAGGCCTTTTATGAACACTAAAACAATGAGGAAATG; TTGGTCATGGGGCAAAGTATCACTTAAAATTGAATTCATCCATTTTTAAAAAACACTTCATGAAAGCAT; TCTGGTGTGAATTGCCATTTTTTTCTTACTGGCTTCTCAATTTTCTTCCTTCTCTGCCCCTACCTAAAA; CATTCTCCTCGGAAATTACATGGTGCTGACCACAAAGTTTCTGGATGTTTTATTAAATATTGTACGTGT; TTACAGTTGGGAATTTAAAATAATACATACACTGGTTGATAAAGGGAAGCTGCAGGACCAAGGTGAAGA; TTGATAGTCCAAATGCTTTTCTTTTTTGAGTTGTATATTTTTTCACACCATCTTAGATATAATTAGGTA; GCTGCTGAAAGGAAAAGTGAATACAGAATTGACGGTATTATTGGAGATTTTTCCTCTGCGTAGAGCCAT; CCAGATCTCTGTATCCTGTTTTGACTAAGTCTTAGGTGGGTTGGGAAGACAGATAATGAAGTAGGCAAA; GAGAAAAGGACCCAAGATAGAGGTTTATATTCAGAAATGGTATATATCAATGACAGCATATCAAACTTC; CTATGGGAAAAAGTCTGGTGGGTGGTCAGCTGACAGATTTCCCATTTAGTAGTCATAGAATACAGAAAT; AGTTTAGGGACATGTATTCATTTTGTTATTTTGAGCATTGATAGGTCAGTATATCTACCTAATCTGTTT; GGTAAGTATAGGATATATAAACCATTACCATTGATCTGTCTTATGCCATAATCTTAAAAAAAATTTGAA; TGCTCTTGAATTTGTATATTCAATAAAGTTATCCTTTTATATTTTTTA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003503.4
• Locus ID: 8317
• Summary: This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008]