CARD15 (NOD2) (NM_022162) Human 3' UTR Clone

SKU SC213503

3' UTR clone of nucleotide-binding oligomerization domain containing 2 (NOD2) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: CARD15
• Synonyms: ACUG; BLAU; BLAUS; CARD15; CD; CLR16.3; IBD1; NLRC2; NOD2B; PSORAS1; YAOS
• ACCN: NM_022162
• Insert Size: 1286 bp
• Sequence Data:
  Insert Sequence

  >SC213503 3’UTR clone of NM_022162 ; The sequence shown below is from the reference sequence of NM_022162. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GGCTGCAGGGACACCAGACTCTTGCTTTGAAGTCTCCGGGAGGATGTTCGTCTCAGTTTGTTTGTGAGC; AGGCTGTGAGTTTGGGCCCCAGAGGCTGGGTGACATGTGTTGGCAGCCTCTTCAAAATGAGCCCTGTCC; TGCCTAAGGCTGAACTTGTTTTCTGGGAACACCATAGGTCACCTTTATTCTGGCAGAGGAGGGAGCATC; AGTGCCCTCCAGGATAGACTTTTCCCAAGCCTACTTTTGCCATTGACTTCTTCCCAAGATTCAATCCCA; GGATGTACAAGGACAGCCCCTCCTCCATAGTATGGGACTGGCCTCTGCTGATCCTCCCAGGCTTCCGTG; TGGGTCAGTGGGGCCCATGGATGTGCTTGTTAACTGAGTGCCTTTTGGTGGAGAGGCCCGGCCTCTCAC; AAAAGACCCCTTACCACTGCTCTGATGAAGAGGAGTACACAGAACACATAATTCAGGAAGCAGCTTTCC; CCATGTCTCGACTCATCCATCCAGGCCATTCCCCGTCTCTGGTTCCTCCCCTCCTCCTGGACTCCTGCA; CACGCTCCTTCCTCTGAGGCTGAAATTCAGAATATTAGTGACCTCAGCTTTGATATTTCACTTACAGCA; CCCCCAACCCTGGCACCCAGGGTGGGAAGGGCTACACCTTAGCCTGCCCTCCTTTCCGGTGTTTAAGAC; ATTTTTGGAAGGGGACACGTGACAGCCGTTTGTTCCCCAAGACATTCTAGGTTTGCAAGAAAAATATGA; CCACACTCCAGCTGGGATCACATGTGGACTTTTATTTCCAGTGAAATCAGTTACTCTTCAGTTAAGCCT; TTGGAAACAGCTCGACTTTAAAAAGCTCCAAATGCAGCTTTAAAAAATTAATCTGGGCCAGAATTTCAA; ACGGCCTCACTAGGCTTCTGGTTGATGCCTGTGAACTGAACTCTGACAACAGACTTCTGAAATAGACCC; ACAAGAGGCAGTTCCATTTCATTTGTGCCAGAATGCTTTAGGATGTACAGTTATGGATTGAAAGTTTAC; AGGAAAAAAAATTAGGCCGTTCCTTCAAAGCAAATGTCTTCCTGGATTATTCAAAATGATGTATGTTGA; AGCCTTTGTAAATTGTCAGATGCTGTGCAAATGTTATTATTTTAAACATTATGATGTGTGAAAACTGGT; TAATATTTATAGGTCACTTTGTTTTACTGTCTTAAGTTTATACTCTTATAGACAACATGGCCGTGAACT; TTATGCTGTAAATAATCAGAGGGGAATAAACTGTTGAGTCAAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022162.3
• Locus ID: 64127
• Summary: This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014]