DEPTOR (NM_022783) Human 3' UTR Clone

SKU SC213241

3' UTR clone of DEP domain containing 6 (DEPDC6) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: DEPTOR
• Synonyms: DEP.6; DEPDC6
• ACCN: NM_022783
• Insert Size: 1244 bp
• Sequence Data:
  Insert Sequence

  >SC213241 3’UTR clone of NM_022783 ; The sequence shown below is from the reference sequence of NM_022783. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; ATGGAAGTCATGGAGGAGTTAGAGTGCTGAGCTCCTGGGCCTCCCAGCCCTCCAGTGGCCTGTGGGTGA; GGGAAGCCAGAATGACACAAAGCAATGCAAAGACAAGATTGCCATGCAAATGGATGGTTTTGGACATAC; GAGTCTTCTCCGCACATACATGTCTAAAGTTGAGTTTTATACACTGAATGTGGAAGAACCGGGTATCAT; ATCTTTTTTAAAAAATGTCAGTGTAGAAAACATTTGGGAAACCATTTTCCTACATGATAGAACTGCCTT; ACTAGATTTCTATTTGTAGCTCTCATTCATTGTTTTTTATCTTAGTTTGCAGAAAGGTGTTGAAATGCT; TCTCTAGCCCAAACAGCGACATGCTAAAGTCCCCTTCTTCAGAGTCAATAGAGTAGTTGTTAAAGGTTT; TAAATTGTACTTTCTCCAAAATTAGCATGCAGCTATTTAATAGGGAATCTAGATTTCACCAAGATTCAA; ATCAAAGCAACATTTAAAGGAATAAGACCTGTTCACTAGCATTTTCAAGGGGGTTCTAAAGCATTCAAG; TGCTTAAAAGCCATAAAAAATGACTTCTTAATTCCTGCCTTTAGTGTCAACTTTTAAGTTAATACAGGT; TTCAATTGTGGCATTAGGAAAAAAAAAAACCTTGTGATGCTATGGTTGGGGGTAGTTAGGGAGAGACTA; CATGAAATTGTGTGCCCCTATTTTCTTTCTGATCCTAAATCATTTTGTTTTATAAATCAGCTATAGCAT; CTTTCTAGAATTAATCCTGAATATGTTGAATGTTAAAATAGAGAAGTTTGTATATACACATAATTAAAA; ATCAACCCTTCTGGCAAGATTTCACTTTGAAGGTGTCTGTTTTTAAGGGAAGGCTAAAACTTTGCTGAT; ATGTGATAAAACTTGAACTCTAAATTGCCTCCTTAGCCTGAATTTTTACTAGCTTTCTAGTGTAACATA; TTCTAAGGACATGACATGCTGCTTATTTGGGGGGATTATTTTTCACCAAAATGATCATCTTGTGTTGTA; ACTTTTCAGCTCACTTGTATATACGCACGTTTTCATTTTTGGTCTAGAAAATAGTGCATTTTGGGGCTC; AGCGTGCAGAGGAAATTCCTCAAAGTGCCAAATTAGGCAGTCAGGAAATACTAAATTCTTCATTCCAAA; TAATGGATCTGAATGATGACGTTAATTTTTTATCATGCATTAACAAAATAAAATATGAGAATTGTACCA; CA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022783.4
• Locus ID: 64798
• Summary: Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.[UniProtKB/Swiss-Prot Function]