SOX2 (NM_003106) Human 3' UTR Clone

SKU SC212702

3' UTR clone of SRY (sex determining region Y)-box 2 (SOX2) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: SOX2
• Synonyms: ANOP3; MCOPS3
• ACCN: NM_003106
• Insert Size: 1152 bp
• Sequence Data:
  Insert Sequence

  >SC212702 3’UTR clone of NM_003106 ; The sequence shown below is from the reference sequence of NM_003106. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AACGGCACACTGCCCCTCTCACACATGTGAGGGCCGGACAGCGAACTGGAGGGGGGAGAAATTTTCAAA; GAAAAACGAGGGAAATGGGAGGGGTGCAAAAGAGGAGAGTAAGAAACAGCATGGAGAAAACCCGGTACG; CTCAAAAAGAAAAAGGAAAAAAAAAAATCCCATCACCCACAGCAAATGACAGCTGCAAAAGAGAACACC; AATCCCATCCACACTCACGCAAAAACCGCGATGCCGACAAGAAAACTTTTATGAGAGAGATCCTGGACT; TCTTTTTGGGGGACTATTTTTGTACAGAGAAAACCTGGGGAGGGTGGGGAGGGCGGGGGAATGGACCTT; GTATAGATCTGGAGGAAAGAAAGCTACGAAAAACTTTTTAAAAGTTCTAGTGGTACGGTAGGAGCTTTG; CAGGAAGTTTGCAAAAGTCTTTACCAATAATATTTAGAGCTAGTCTCCAAGCGACGAAAAAAATGTTTT; AATATTTGCAAGCAACTTTTGTACAGTATTTATCGAGATAAACATGGCAATCAAAATGTCCATTGTTTA; TAAGCTGAGAATTTGCCAATATTTTTCAAGGAGAGGCTTCTTGCTGAATTTTGATTCTGCAGCTGAAAT; TTAGGACAGTTGCAAACGTGAAAAGAAGAAAATTATTCAAATTTGGACATTTTAATTGTTTAAAAATTG; TACAAAAGGAAAAAATTAGAATAAGTACTGGCGAACCATCTCTGTGGTCTTGTTTAAAAAGGGCAAAAG; TTTTAGACTGTACTAAATTTTATAACTTACTGTTAAAAGCAAAAATGGCCATGCAGGTTGACACCGTTG; GTAATTTATAATAGCTTTTGTTCGATCCCAACTTTCCATTTTGTTCAGATAAAAAAAACCATGAAATTA; CTGTGTTTGAAATATTTTCTTATGGTTTGTAATATTTCTGTAAATTTATTGTGATATTTTAAGGTTTTC; CCCCCTTTATTTTCCGTAGTTGTATTTTAAAAGATTCGGCTCTGTATTATTTGAATCAGTCTGCCGAGA; ATCCATGTATATATTTGAACTAATATCATCCTTATAACAGGTACATTTTCAACTTAAGTTTTTACTCCA; TTATGCACAGTTTGAGATAAATAAATTTTTGAAATATGGACACTGAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003106.4
• Locus ID: 6657
• Summary: This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]