FAK (PTK2) (NM_153831) Human 3' UTR Clone

SKU SC212185

3' UTR clone of PTK2 protein tyrosine kinase 2 (PTK2) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: FAK
• Synonyms: FADK; FADK 1; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71
• ACCN: NM_153831
• Insert Size: 1596 bp
• Sequence Data:
  Insert Sequence

  >SC212185 3’UTR clone of NM_153831 ; The sequence shown below is from the reference sequence of NM_153831. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTTTGAAG; ATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTC; CAACTTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGG; TTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTT; TCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATAC; GTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAG; GAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTAT; TATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAG; AATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTG; CCTTTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTC; ATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGTTATTTCTCTTGGAAGCAGAAATGC; TTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTAT; ATAATTTATAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCT; TGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTACAC; CATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCA; GGAAGAATATAATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTG; TAATATCCTTTCTTTTGGTTCTGTTTCTTCAGATGGCTTTTCAGGTGACTTGTCAGATAAGATAACATA; CAAGAGAGTTCCCAATAATTATCAGGAAGCCTCTCACTAATTAGTTTCTTTTTTTTTATTTAATCTTCT; AAACAAGTCAAGGATGTTACCACAGAAGGGTAGCGTGGCGTAAGTTAACCTTTTCATTCCATCGTCACC; TATTAAATGCAGAGCTCAGGATGGAAGTAGCAATGGTCTTCAATGAAAGAACCTTCCCCTGCGCAGGGT; CTCACTATTCCGCTAACTCGGACTCAAAGCATAATTCACTTGTCAAGGAAATTGTTAGCATTTCCCAGG; CCCACCCTAGTCTTGCTAGAATCTCTGTGAGCCACAGAGCAGGGAGCAAATAGAACTGAGATCTCCAGC; ACTAGGGACTCAGACTCCAAAGGGAGGGATTTGAGTGAATTTTTTCCAGGTAAATACATGCTCTTATGT; GCAAAAGCA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153831.4
• Locus ID: 5747
• Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]