RASSF8 (NM_007211) Human 3' UTR Clone

SKU SC210298

3' UTR clone of Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 (RASSF8) transcript variant 4 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: RASSF8
• Synonyms: C12orf2; HOJ1
• ACCN: NM_007211
• Insert Size: 846 bp
• Sequence Data:
  Insert Sequence

  >SC210298 3’UTR clone of NM_007211 ; The sequence shown below is from the reference sequence of NM_007211. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CTTTCTGATAAGCAGGAGTGTAAAGATTAGATATCACACCAAAAGCTCAGGCAAGAAAAGCAAAAATGA; ACAAGTGGGACTACATTAAACCCAAAACTCCTGCACAACAAAGGAAACGATCACCAAAATGAAAAGGTG; GACTGTAGATTGGGAGAAAATATTTGCAAACCGTTATCTGGTAAGGGTTTAATATCCAAACTATATAAG; GAACTCACAGAACTTAAAGAAAACAAATAATCCAATTAAAAATGGTCAAAGGGGCTGGGCATGGTGGCT; CACGCCTGTAATCTCAGCACTTTGGGAGGCCGAGGTGGGAGAATCACAAGGTCAGGAGACTGAGACCAT; CCTGGCTAACACCATGATGGGCTAACACCCATCTCTACTAAAAATATGAAACATTAGCCAGGTGTGGTG; GCACGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGAAGGATTGTTTGAACCCGGGAGGTGGAG; GTTGCAGTGAGCCGAGATCGCATTACTGCACTCCAGCCTGGGTAACAGAGTGAGACTCCATCTCAAAAA; AAAAAAAAATAGATATTTTTCCAAAGAAGACATAAAAACAGCCAACAGGTATATGAAAAAGTGCTCAAC; ATCACTGATCAGCAGGGAAATGCAAATCAAAGCCACAATGAGATACCACCTCACACCTGTTAGGTTACA; TCTGCTATTGTCAAAAAGATAAGAGATAGCAAGTGTCAGCAAGGGTCTGGAGAAAAGGGAATTTTTGTA; TACTGTTGGTGGGAATGTAAATCAGTACAGCTATTGTGGAAAACAGTGTAGAGGTTTCTCAAAAAATTA; AAACTATCATACAACCCA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007211.5
• Locus ID: 11228
• Summary: This gene encodes a member of the Ras-assocation domain family (RASSF) of tumor suppressor proteins. This gene is essential for maintaining adherens junction function in epithelial cells and has a role in epithelial cell migration. It is a lung tumor suppressor gene candidate. A chromosomal translocation t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the FBLN1 gene is found in a complex type of synpolydactyly. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]