Fatty Acid Synthase (FASN) (NM_004104) Human 3' UTR Clone

SKU SC210244

3' UTR clone of fatty acid synthase (FASN) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Fatty Acid Synthase
• Synonyms: FAS; OA-519; SDR27X1
• ACCN: NM_004104
• Insert Size: 835 bp
• Sequence Data:
  Insert Sequence

  >SC210244 3’UTR clone of NM_004104 ; The sequence shown below is from the reference sequence of NM_004104. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GAGCCACGCGTGAGCGTGCGGGAGGGCTAGGCCCGTGCCCCCGCCTGCCACCGGAGGTCACTCCACCAT; CCCCACCCCACCCCACCCCACCCCCGCCATGCAACGGGATTGAAGGGTCCTGCCGGTGGGACCCTGTCC; GGCCCAGTGCCACTGCCCCCCGAGGCTGCTAGATGTAGGTGTTAGGCATGTCCCACCCACCCGCCGCCT; CCCACGGCACCTCGGGGACACCAGAGCTGCCGACTTGGAGACTCCTGGTCTGTGAAGAGCCGGTGGTGC; CCGTGCCCGCAGGAACTGGGCTGGGCCTCGTGCGCCCGTGGGGTCTGCGCTTGGTCTTTCTGTGCTTGG; ATTTGCATATTTATTGCATTGCTGGTAGAGACCCCCAGGCCTGTCCACCCTGCCAAGACTCCTCAGGCA; GCGTGTGGGTCCCGCACTCTGCCCCCATTTCCCCGATGTCCCCTGCGGGCGCGGGCAGCCACCCAAGCC; TGCTGGCTGCGGCCCCCTCTCGGCCAGGCATTGGCTCAGCCCGCTGAGTGGGGGGTCGTGGGCCAGTCC; CCGAGGAGCTGGGCCCCTGCACAGGCACACAGGGCCCGGCCACACCCAGCGGCCCCCCGCACAGCCACC; CGTGGGGTGCTGCCCTTATGCCCGGCGCCGGGCACCAACTCCATGTTTGGTGTTTGTCTGTGTTTGTTT; TTCAAGAAATGATTCAAATTGCTGCTTGGATTTTGAAATTTACTGTAACTGTCAGTGTACACGTCTGGA; CCCCGTTTCATTTTTACACCAATTTGGTAAAAATGCTGCTCTCAGCCTCCCACAATTAAACCGCATGTG; ATCTCCA; AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC; CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC;
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004104.5
• Locus ID: 2194
• Summary: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]