PARP1 (NM_001618) Human 3' UTR Clone

SKU SC209859

3' UTR clone of poly (ADP-ribose) polymerase 1 (PARP1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PARP1
• Synonyms: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL
• ACCN: NM_001618
• Insert Size: 799 bp
• Sequence Data:
  Insert Sequence

  >SC209859 3’UTR clone of NM_001618 ; The sequence shown below is from the reference sequence of NM_001618. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AAATTCAATTTTAAGACCTCCCTGTGGTAATTGGGAGAGGTAGCCGAGTCACACCCGGTGGCTCTGGTA; TGAATTCACCCGAAGCGCTTCTGCACCAACTCACCTGGCCGCTAAGTTGCTGATGGGTAGTACCTGTAC; TAAACCACCTCAGAAAGGATTTTACAGAAACGTGTTAAAGGTTTTCTCTAACTTCTCAAGTCCCTTGTT; TTGTGTTGTGTCTGTGGGGAGGGGTTGTTTTGGGGTTGTTTTTGTTTTTTCTTGCCAGGTAGATAAAAC; TGACATAGAGAAAAGGCTGGAGAGAGATTCTGTTGCATAGACTAGTCCTATGGAAAAAACCAAGCTTCG; TTAGAATGTCTGCCTTACTGGTTTCCCCAGGGAAGGAAAAATACACTTCCACCCTTTTTTCTAAGTGTT; CGTCTTTAGTTTTGATTTTGGAAAGATGTTAAGCATTTATTTTTAGTTAAAAATAAAAACTAATTTCAT; ACTATTTAGATTTTCTTTTTTATCTTGCACTTATTGTCCCCTTTTTAGTTTTTTTTGTTTGCCTCTTGT; GGTGAGGGGTGTGGGAAGACCAAAGGAAGGAACGCTAACAATTTCTCATACTTAGAAACAAAAAGAGCT; TTCCTTCTCCAGGAATACTGAACATGGGAGCTCTTGAAATATGTAGTATTAAAAGTTGCATTTGAAATT; CTTGACTTTCTTATGGGCACTTTTGTCTTCCAAATTAAAACTCTACCACAAATATACTTACCCAAGGGC; TAATAGTAATACTCGATTAAAAATGCAGATGCCTTCTCTA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001618.4
• Locus ID: 142
• Summary: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]