PARP1 (NM_001618) Human 3' UTR Clone

CAT#: SC209859

3' UTR clone of poly (ADP-ribose) polymerase 1 (PARP1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PARP1
• Synonyms: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL
• ACCN: NM_001618
• Insert Size: 799 bp
• Sequence Data:
  Insert Sequence

  >SC209859 3’UTR clone of NM_001618
  The sequence shown below is from the reference sequence of NM_001618. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAATTCAATTTTAAGACCTCCCTGTGGTAATTGGGAGAGGTAGCCGAGTCACACCCGGTGGCTCTGGTA
  TGAATTCACCCGAAGCGCTTCTGCACCAACTCACCTGGCCGCTAAGTTGCTGATGGGTAGTACCTGTAC
  TAAACCACCTCAGAAAGGATTTTACAGAAACGTGTTAAAGGTTTTCTCTAACTTCTCAAGTCCCTTGTT
  TTGTGTTGTGTCTGTGGGGAGGGGTTGTTTTGGGGTTGTTTTTGTTTTTTCTTGCCAGGTAGATAAAAC
  TGACATAGAGAAAAGGCTGGAGAGAGATTCTGTTGCATAGACTAGTCCTATGGAAAAAACCAAGCTTCG
  TTAGAATGTCTGCCTTACTGGTTTCCCCAGGGAAGGAAAAATACACTTCCACCCTTTTTTCTAAGTGTT
  CGTCTTTAGTTTTGATTTTGGAAAGATGTTAAGCATTTATTTTTAGTTAAAAATAAAAACTAATTTCAT
  ACTATTTAGATTTTCTTTTTTATCTTGCACTTATTGTCCCCTTTTTAGTTTTTTTTGTTTGCCTCTTGT
  GGTGAGGGGTGTGGGAAGACCAAAGGAAGGAACGCTAACAATTTCTCATACTTAGAAACAAAAAGAGCT
  TTCCTTCTCCAGGAATACTGAACATGGGAGCTCTTGAAATATGTAGTATTAAAAGTTGCATTTGAAATT
  CTTGACTTTCTTATGGGCACTTTTGTCTTCCAAATTAAAACTCTACCACAAATATACTTACCCAAGGGC
  TAATAGTAATACTCGATTAAAAATGCAGATGCCTTCTCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001618.4
• Summary: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
• Locus ID: 142
• MW: 30.9