PFKM (NM_000289) Human 3' UTR Clone
CAT#: SC209129
3' UTR clone of phosphofructokinase muscle (PFKM) for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | PFKM |
Synonyms | ATP-PFK; GSD7; PFK-1; PFK-A; PFK1; PFKA; PFKX; PPP1R122 |
ACCN | NM_000289 |
Insert Size | 726 bp |
Sequence Data |
>SC209129 3’UTR clone of NM_000289
The sequence shown below is from the reference sequence of NM_000289. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC CGGAAGCGGTCCGGGGAAGCTGCCGTCTAAACCTCTCTGGAGTGAGGGGAATAGATTACCTGATCATGG TCAGCTCACACCCTAATAAGTCCACATCTTCTCAGTGTTTTAGCTGTTTTTTTCATTAGGTTTCCTTTT ATTCTGTACCTTGCAGCCATGACCAGTTCTGGCCAGGAGCTGGAGGAGCAGGCAGTGGGTGGGAGCTCC TTTTAGGTAGAATTTAACATGACTTCTGCCCCAGCTTTATCTGTCACACAAGGCTGGGCACCTCTAGTG CTACTGCTAGATATCACTTACTCAGTTAGAATTTTCCTAAAAATAAGCTTTATTTATTTCTTTGTGATA ACAAAGAGTCTTGGTTCCTCTACTACTTTTACTACAGTGACAAATTGTAACTACACTAATAAATGCCAA CTGGTCACTGTGCTTTTGCTTCTCCTGTTATCATCTTCCTAAGTGGAATGTAATACTGTCAGCCCCATG TATCAGACACTTGTCTGATGAAGCAGTAAAGACGTTAAGGGTATCACAGGGGGTGGAGGAAGGGATTAT CTCTAGTACACTACTTGCTGGCTGTCTGAAAAATTGTCACTGCCAAACTCTAAAAACAGTTCTAAATAG TGACTGAGAAGGTTTGTTGCTGGAGTCAGGGAATAAGGCAGCCAAATACTCTTTGCACAGTTCTTTAGT GGGAAGAGAAATTAACAATAAATATCAAGCACTGTG ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_000289.6 |
Summary | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009] |
Locus ID | 5213 |
MW | 26.7 |
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