PFKM (NM_000289) Human 3' UTR Clone

SKU SC209129

3' UTR clone of phosphofructokinase muscle (PFKM) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PFKM
• Synonyms: ATP-PFK; GSD7; PFK-1; PFK-A; PFK1; PFKA; PFKX; PPP1R122
• ACCN: NM_000289
• Insert Size: 726 bp
• Sequence Data:
  Insert Sequence

  >SC209129 3’UTR clone of NM_000289 ; The sequence shown below is from the reference sequence of NM_000289. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CGGAAGCGGTCCGGGGAAGCTGCCGTCTAAACCTCTCTGGAGTGAGGGGAATAGATTACCTGATCATGG; TCAGCTCACACCCTAATAAGTCCACATCTTCTCAGTGTTTTAGCTGTTTTTTTCATTAGGTTTCCTTTT; ATTCTGTACCTTGCAGCCATGACCAGTTCTGGCCAGGAGCTGGAGGAGCAGGCAGTGGGTGGGAGCTCC; TTTTAGGTAGAATTTAACATGACTTCTGCCCCAGCTTTATCTGTCACACAAGGCTGGGCACCTCTAGTG; CTACTGCTAGATATCACTTACTCAGTTAGAATTTTCCTAAAAATAAGCTTTATTTATTTCTTTGTGATA; ACAAAGAGTCTTGGTTCCTCTACTACTTTTACTACAGTGACAAATTGTAACTACACTAATAAATGCCAA; CTGGTCACTGTGCTTTTGCTTCTCCTGTTATCATCTTCCTAAGTGGAATGTAATACTGTCAGCCCCATG; TATCAGACACTTGTCTGATGAAGCAGTAAAGACGTTAAGGGTATCACAGGGGGTGGAGGAAGGGATTAT; CTCTAGTACACTACTTGCTGGCTGTCTGAAAAATTGTCACTGCCAAACTCTAAAAACAGTTCTAAATAG; TGACTGAGAAGGTTTGTTGCTGGAGTCAGGGAATAAGGCAGCCAAATACTCTTTGCACAGTTCTTTAGT; GGGAAGAGAAATTAACAATAAATATCAAGCACTGTG; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000289.6
• Locus ID: 5213
• Summary: Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]