PRKRA (NM_003690) Human 3' UTR Clone

SKU SC208870

3' UTR clone of protein kinase interferon-inducible double stranded RNA dependent activator (PRKRA) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PRKRA
• Synonyms: DYT16; HSD14; PACT; RAX
• ACCN: NM_003690
• Insert Size: 713 bp
• Sequence Data:
  Insert Sequence

  >SC208870 3’UTR clone of NM_003690 ; The sequence shown below is from the reference sequence of NM_003690. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TATTTAAAGATAATAGCAGAAAGAAAGTAAATCTGGAGCAACTTAAAAAATCTTTCAGTAGCACATAAA; AAGTTCCCCTCTGGCCCCTTCCCAAGTAAAACTTTTACCGTAGTGTTTATGTCTTGTTTCTAAATCTCT; TCATAGATTCCATCAACACTCCAGATTTAATTATCTCCTCATAGTTGTTATTAAGCTCTTTTTAATGGC; TTCAACTTTGTATCAGTATACTGTATTTATAAACTTTGTACCACAAGAGAGAGTGTAGCACCCATTTTA; CAGTGCCATGCACATCAGAGAAAGAAACTGCATGTTTGTTGTTGATGATGAAATAAAAATGCTAGCGAC; AGTCTTTCTTACTGGTGCTTAAGCTCTTCTTTGCACAAAGCTTTATAAAGGGAATTCAAAGGAAGCCCT; TTAGAATTAGAGTCTTGAGGGACAGCACTAACAGGCCTTTATTAAGTATGATTGATTGTTAAATTTCAG; GGAACATGATTGGTCTGCTGTGTATTTGAATTCATGTAACAAAGAACTGTTACGATGGGATTCTGCTCA; TTTTATTAAAAAGCTACTGACTTGACTGTCATCCTGTTCTTGTTAGCCATTGTGAATAAGATTTTAATG; TTGATAATTCTGTTATTTACATATCTCTAATTTACTTTGAAATTCAAAGGTGAAAATAAAAAATGATGG; CCTAAGTAAAATTTACAAACATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003690.5
• Locus ID: 8575
• Summary: This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]