EGLN2 (NM_053046) Human 3' UTR Clone

SKU SC207831

3' UTR clone of egl nine homolog 2 (C. elegans) (EGLN2) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: EGLN2
• Synonyms: EIT-6; EIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1
• ACCN: NM_053046
• Insert Size: 600 bp
• Sequence Data:
  Insert Sequence

  >SC207831 3’UTR clone of NM_053046 ; The sequence shown below is from the reference sequence of NM_053046. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CCTGTATCACAGCCGCCTACGCCCACCTAGTGGCCAGTCCCAGAGCCGCATGGCAGACAGCTTAAATGA; CTTCAGGAGAGCCCTGGGCCTGTGCTGGCTGCTCCTTCCCTGCCACCGCTGCTGCTTCTGACTTTGCCT; CTGTCCTGCCTGGTGTGGAGGGCTCTGTCTGTTGCTGAGGACCAAGGAGGAGAAGAGACCTTTGCTGCC; CCATCATGGGGGCTGGGGTTGTCACCTGGACAGGGGGCAGCCGTGGAGGCCACCGTTACCAACTGAAGC; TGGGGGCCTGGGTCCTACCCTGTCTGGTCATGACCCCATTAGGTATGGAGAGCTGGGAGGAGGCATTGT; CACTTCCCACCAGGATGCAGGACTTGGGGTTGAGGTGAGTCATGGCCTCTTGCTGGCAATGGGGTGGGA; GGAGTACCCCCAAGTCCTCTCACTCCTCCAGCCTGGAATGTGAAGTGACTCCCCAACCCCTTTGGCCAT; GGCAGGCACCTTTTGGACTGGGCTGCCACTGCTTGGGCAGAGTAAAAGGTGCCAGGAGGAGCATGGGTG; TGGAAGTCCTGTCAGCCAAGAAATAAAAGTTTACCTCAGAGCTGCACA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_053046.4
• Locus ID: 112398
• Summary: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]