Nucleolin (NCL) (NM_005381) Human 3' UTR Clone

CAT#: SC206187

3' UTR clone of nucleolin (NCL) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NCL
• Synonyms: C23; Nsr1
• ACCN: NM_005381
• Insert Size: 1690 bp
• Sequence Data:
  Insert Sequence

  >SC206187 3’UTR clone of NM_005381
  The sequence shown below is from the reference sequence of NM_005381. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCACAAGGAAAGAAGACGAAGTTTGAATAGCTTCTGTCCCTCTGCTTTCCCTTTTCCATTTGAAAGAAA
  GGACTCTGGGGTTTTTACTGTTACCTGATCAATGACAGAGCCTTCTGAGGACATTCCAAGACAGTATAC
  AGTCCTGTGGTCTCCTTGGAAATCCGTCTAGTTAACATTTCAAGGGCAATACCGTGTTGGTTTTGACTG
  GATATTCATATAAACTTTTTAAAGAGTTGAGTGATAGAGCTAACCCTTATCTGTAAGTTTTGAATTTAT
  ATTGTTTCATCCCATGTACAAAACCATTTTTTCCTACAAATAGTTTGGGTTTTGTTGTTGTTTCTTTTT
  TTTGTTTTGTTTTTGTTTTTTTTTTTTTTGCGTTCGTGGGGTTGTAAAAGAAAAGAAAGCAGAATGTTT
  TATCATGGTTTTTGCTTCAGCGGCTTTAGGACAAATTAAAAGTCAACTCTGGTGCCAGACGTGTTACTT
  CCTAAAGAGTGTTTCCCCTGGAATGTCACTGGAGAGCATGGCAAAGCCAGCTCTGCCACTTGCTTCACC
  CATCCCAATGGAAATGGCTTAGTGCGTGTTTCCAGTATCCCAGCCCTAACTAACTTGGTTGAAATGCTG
  GTGAGGGGACCTGCTCCTGCAGCCCTGGTGCTGACTTGAAGGCTGCTGCAGCTTCTCCTACTTTTAGCA
  GGTCTGAGGATTATGTCCTGAAGACCACTCTGGAAAGAGGTGCAGGAACAGATTAGTCAGGTTTCCTAG
  GACAAGGAAGAGCTTCAGGGAAGAGCAGTGGCTAACTCCTGTAATCCCAACACTTGGGGAGGCCGAGGC
  AGGCAGATCAACTGAGGTCAGGAGTTGAAGACCAGCCTGGCCAACATGGTGAAAGCCCATCTCTACTAA
  AAATACAAAAATTAGCTGGGCATGGTGGTGTACTCCTGTAGTCCCAGCTACTCAGGAGGCTGAAGCGGG
  AGAGTCACGTGAACCCGGGAAGCAGAGTGAGCTGAGCACACACTACTATACTCCAGGCTGGGTAACAAA
  GCGAGACTCCCATCTCCCAAAAAGCAGTTCTGGAATAGAACTCACGCTAGATGGATAGACCAGTGGACA
  CTTTGGAACCTTGGGGCTGGGGAGGAAACTGCCCATCCAGTAAACCCCCAAAAAGCCATTTGTTCTGCA
  CTACGTATATTGCTTATTCTTTCTGGTCTTAAGTACTTGCCTCTCAACCTCCCTTTTTAGTAAAAGACA
  AGGCCACGTGAGAGGCGGGACTATCAACATTGTGATGAATTTACTTGAAACCCAGTGCCCAAAATCAAT
  GTAGGTAGCCAAGTCCAAAAACCTGTTCTAGTCCAACTAGTGAAATCAAACTGTGATACTTGGATAAGC
  TTAGAAGGAAACGTGAAGAATACGTAGCTGCTTTGGGTTTACTCTGGTTCAGTTGGGCTGTTGAAATCT
  TAACATCCTTGGGCTTATCACCTACTGCTTGTCAGCCCTGTTCCATGTCCAGGGGATGGGGGTGGTGAC
  AATCCAGTTCCAAGACCCTCATGCTCTAGAGAGGAAGGTGGCCAGCCAGGGTTGTAACTACGATGAAAA
  AGCAGTGGGAGGGTCTCCTATGAGGCAAGCCTAAGGACAAAAAGGAAGGCCTTGCAGCCTGTATTCTGG
  ATAAGGAATTAAAAGCTCAGTTAATTGAAGCCCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005381.3
• Summary: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
• Locus ID: 4691
• MW: 63.9