Nucleolin (NCL) (NM_005381) Human 3' UTR Clone

SKU SC206187

3' UTR clone of nucleolin (NCL) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Nucleolin
• Synonyms: C23; Nsr1
• ACCN: NM_005381
• Insert Size: 1690 bp
• Sequence Data:
  Insert Sequence

  >SC206187 3’UTR clone of NM_005381 ; The sequence shown below is from the reference sequence of NM_005381. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CCACAAGGAAAGAAGACGAAGTTTGAATAGCTTCTGTCCCTCTGCTTTCCCTTTTCCATTTGAAAGAAA; GGACTCTGGGGTTTTTACTGTTACCTGATCAATGACAGAGCCTTCTGAGGACATTCCAAGACAGTATAC; AGTCCTGTGGTCTCCTTGGAAATCCGTCTAGTTAACATTTCAAGGGCAATACCGTGTTGGTTTTGACTG; GATATTCATATAAACTTTTTAAAGAGTTGAGTGATAGAGCTAACCCTTATCTGTAAGTTTTGAATTTAT; ATTGTTTCATCCCATGTACAAAACCATTTTTTCCTACAAATAGTTTGGGTTTTGTTGTTGTTTCTTTTT; TTTGTTTTGTTTTTGTTTTTTTTTTTTTTGCGTTCGTGGGGTTGTAAAAGAAAAGAAAGCAGAATGTTT; TATCATGGTTTTTGCTTCAGCGGCTTTAGGACAAATTAAAAGTCAACTCTGGTGCCAGACGTGTTACTT; CCTAAAGAGTGTTTCCCCTGGAATGTCACTGGAGAGCATGGCAAAGCCAGCTCTGCCACTTGCTTCACC; CATCCCAATGGAAATGGCTTAGTGCGTGTTTCCAGTATCCCAGCCCTAACTAACTTGGTTGAAATGCTG; GTGAGGGGACCTGCTCCTGCAGCCCTGGTGCTGACTTGAAGGCTGCTGCAGCTTCTCCTACTTTTAGCA; GGTCTGAGGATTATGTCCTGAAGACCACTCTGGAAAGAGGTGCAGGAACAGATTAGTCAGGTTTCCTAG; GACAAGGAAGAGCTTCAGGGAAGAGCAGTGGCTAACTCCTGTAATCCCAACACTTGGGGAGGCCGAGGC; AGGCAGATCAACTGAGGTCAGGAGTTGAAGACCAGCCTGGCCAACATGGTGAAAGCCCATCTCTACTAA; AAATACAAAAATTAGCTGGGCATGGTGGTGTACTCCTGTAGTCCCAGCTACTCAGGAGGCTGAAGCGGG; AGAGTCACGTGAACCCGGGAAGCAGAGTGAGCTGAGCACACACTACTATACTCCAGGCTGGGTAACAAA; GCGAGACTCCCATCTCCCAAAAAGCAGTTCTGGAATAGAACTCACGCTAGATGGATAGACCAGTGGACA; CTTTGGAACCTTGGGGCTGGGGAGGAAACTGCCCATCCAGTAAACCCCCAAAAAGCCATTTGTTCTGCA; CTACGTATATTGCTTATTCTTTCTGGTCTTAAGTACTTGCCTCTCAACCTCCCTTTTTAGTAAAAGACA; AGGCCACGTGAGAGGCGGGACTATCAACATTGTGATGAATTTACTTGAAACCCAGTGCCCAAAATCAAT; GTAGGTAGCCAAGTCCAAAAACCTGTTCTAGTCCAACTAGTGAAATCAAACTGTGATACTTGGATAAGC; TTAGAAGGAAACGTGAAGAATACGTAGCTGCTTTGGGTTTACTCTGGTTCAGTTGGGCTGTTGAAATCT; TAACATCCTTGGGCTTATCACCTACTGCTTGTCAGCCCTGTTCCATGTCCAGGGGATGGGGGTGGTGAC; AATCCAGTTCCAAGACCCTCATGCTCTAGAGAGGAAGGTGGCCAGCCAGGGTTGTAACTACGATGAAAA; AGCAGTGGGAGGGTCTCCTATGAGGCAAGCCTAAGGACAAAAAGGAAGGCCTTGCAGCCTGTATTCTGG; ATAAGGAATTAAAAGCTCAGTTAATTGAAGCCCA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005381.3
• Locus ID: 4691
• Summary: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]