DGKA (NM_001345) Human 3' UTR Clone

SKU SC203656

3' UTR clone of diacylglycerol kinase alpha 80kDa (DGKA) transcript variant 3 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: DGKA
• Synonyms: DAGK; DAGK1; DGK-alpha
• ACCN: NM_001345
• Insert Size: 285 bp
• Sequence Data:
  Insert Sequence

  >SC203656 3’UTR clone of NM_001345 ; The sequence shown below is from the reference sequence of NM_001345. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TCCACCAATTTCTTTGGCTTCTTGAGCTAAGGGGGACACCCTTGGCCTCCAAGCCAGCCTTGAACCCAC; CTCCCTGTCCCTGGACTCTACTCCCGAGGCTCTGTACATTGCTGCCACATACTCCTGCCAGCTTGGGGG; AGTGTTCCTTCACCCTCACAGTATTTATTATCCTGCACCACCTCACTGTTCCCCATGCGCACACACATA; CACACACCCCAAAACACATACATTGAAAGTGCCTCATCTGAATAAAATGACTTGTGTTTCCCCTTTGGG; ATCTGCTAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001345.5
• Locus ID: 1606
• Summary: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]