3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal
Colorimetric
Curve Range
62.5-4000pg/mL
Sample Type
Human serum, plasma, cell lysates and other biological fluids.
Sample Volume
100µl
Specificity
This kit is used for quantitative detection of PIP5K3
Sensitivity
22pg/mL
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Interference
No significant interference observed with available related molecules.
Components
PIP5K3 Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
PIP5K3 Standard Protein | 0.1 mL
PIP5K3 Detection Antibody | 0.12 mL
1x SA-HRP Conjugate | 0.12 mL
Assay Buffer | 40 mL
Sample Diluent | 30 mL
Wash Buffer Concentrate 20X | 60 mL
TMB Substrate | 14 mL
Stop Solution | 14 mL
Background
PIP5K3 also known as PIKFYVE is a FYVE finger-containing phosphoinositide kinase with a significant role in the phosphorylation of phosphatidylinositol 3-phosphate to phosphatidylinositol (3, 5)-bisphosphate. It is predominantly expressed in the cytoplasm of a variety of cell types. Its enzymatic activity is essential for the maintenance of phosphatidylinositol 3, 5-bisphosphate levels influencing membrane trafficking and endosomal function. PIP5K3 functions as an important component in regulating endosomal dynamics. It is part of a complex with VAC14 and FIG4 which plays a significant role in endosomal membrane trafficking lysosome biogenesis and autophagy. The protein's expression aids in maintaining cellular homeostasis and modulating organelle identity within the endosomal-lysosomal system which is critical for normal cellular operations.
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in shipping.