Human PIP5K3 (PIKFYVE) ELISA Kit 1 x 96

SKU
EA200075
Human PIP5K3 (PIKFYVE) ELISA Kit 1 x 96
$600.00
2 Weeks*
Specifications
Product Data
Species Human
Format 8x12 divisible strips
Assay Type microplate ELISA
Assay Length 3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5-4000pg/mL
Sample Type Human serum, plasma, cell lysates and other biological fluids.
Sample Volume 100µl
Specificity This kit is used for quantitative detection of PIP5K3
Sensitivity 22pg/mL
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • PIP5K3 Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
  • PIP5K3 Standard Protein | 0.1 mL
  • PIP5K3 Detection Antibody | 0.12 mL
  • 1x SA-HRP Conjugate | 0.12 mL
  • Assay Buffer | 40 mL
  • Sample Diluent | 30 mL
  • Wash Buffer Concentrate 20X | 60 mL
  • TMB Substrate | 14 mL
  • Stop Solution | 14 mL
Background PIP5K3 also known as PIKFYVE is a FYVE finger-containing phosphoinositide kinase with a significant role in the phosphorylation of phosphatidylinositol 3-phosphate to phosphatidylinositol (3, 5)-bisphosphate. It is predominantly expressed in the cytoplasm of a variety of cell types. Its enzymatic activity is essential for the maintenance of phosphatidylinositol 3, 5-bisphosphate levels influencing membrane trafficking and endosomal function. PIP5K3 functions as an important component in regulating endosomal dynamics. It is part of a complex with VAC14 and FIG4 which plays a significant role in endosomal membrane trafficking lysosome biogenesis and autophagy. The protein's expression aids in maintaining cellular homeostasis and modulating organelle identity within the endosomal-lysosomal system which is critical for normal cellular operations.
Gene Symbol PIKFYVE
Locus ID 200576
Standard Curve
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.