3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal
Colorimetric
Curve Range
0.156-10ng/ml
Sample Type
Human serum, plasma, and other biological fluids.
Sample Volume
100µl
Specificity
This kit is used for quantitative detection of AKT2
Sensitivity
60pg/ml
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Interference
No significant interference observed with available related molecules.
Components
AKT2 Monoclonal Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
Human AKT2 Standard (500ng/ml) | 0.1 mL
100x Biotin conjugated AKT2 Detection Antibody | 0.12 mL
100x SA-HRP Conjugate | 0.12 mL
Assay Buffer | 40 mL
Sample Diluent | 20 mL
Wash Buffer Concentrate 20X | 60 mL
TMB Substrate | 12 mL
Stop Solution | 12 mL
Plate Sealer | 3 pieces
Background
AKT2, also known as RAC-beta serine/threonine-protein kinase, is an enzyme that in humans is encoded by the AKT2 gene. It influences metabolite storage as part of the insulin signal transduction pathway. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) that belong to the AKT subfamily. AKT represents an important signaling hub, modulating many processes including metabolism, proliferation, cell survival, growth and angiogenesis by phosphorylating a range of downstream substrates in response to growth factor stimulation. Akt2 is associated with the development of human cancers. Amplification or high levels of expression of Akt2 are frequently found in human pancreatic, lung, colorectal, ovarian, and breast cancers, and high Akt2 expression levels are positively correlated with the aggressiveness of cancer or poor survival rates in colorectal, ovarian, and breast cancers. AKT2 constitutes a prognostic marker of poor clinical outcomes in breast cancer, ovarian cancer, lung adenocarcinoma, and hepatocellular carcinoma.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.