Human alpha 1 Fetoprotein (AFP) ELISA Kit 1 x 96

SKU EA200031

Human alpha 1 Fetoprotein (AFP) ELISA Kit 1 x 96

Product Data

• Species: Human
• Format: 8x12 divisible strips
• Assay Type: Sandwich
• Assay Length: 3.5 hours incubations; 0.5 hour washing and analyzing samples
• Signal: Colorimetric
• Sample Type: Human serum, plasma and other biological fluids.
• Sample Volume: 100µl
• Specificity: This kit is used for quantitative detection of AFP
• Sensitivity: 0.13ng/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • AFP Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
  • Recombinant Human AFP Standard (1.25µg/ml) | 0.1 mL
  • 100x Biotin conjugated AFP Detection Antibody | 0.12 mL
  • 100x SA-HRP Conjugate | 0.12 mL
  • Assay Buffer | 30 mL
  • Standard & Sample Diluent | 30 mL
  • Wash Buffer Concentrate 20X | 60 mL
  • TMB Substrate | 12 mL
  • Stop Solution | 12 mL
  • Plate Sealer | 3 pieces
• Background: Alpha-fetoprotein (AFP) is a protein encoded by the AFP gene. AFP is a glycoprotein of 591 amino acids, found in monomeric, dimeric and trimeric forms. AFP is a major plasma protein produced by the yolk sac and the fetal liver during fetal development. AFP binds to copper, nickel, fatty acids and bilirubin. Serum AFP level is elevated in people with developmental birth defects such as omphalocele, gastroschisis and neural tube defects, hepatocellular carcinoma, metastatic disease affecting the liver, yolk sac tumor, nonseminomatous germ cell tumors, and ataxia telangiectasia. AFP has been used as a biomarker to these diseases.
• Gene Symbol: AFP
• Locus ID: 174
• Shipping: Blue Ice