Human MDC (CCL22) ELISA KIT (1 x 96 wells)

SKU EA102899

The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human CCL22 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CCL22 tissue specificity: Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. .

Product Data

• Species: Human
• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human MDC
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31.2pg/ml-2000pg/ml
• Specificity: This kit is used for quantitative detection of Human MDC
• Sensitivity: <1pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 10ng/tube
• Background: Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.
• Gene Symbol: CCL22
• Locus ID: 6367
• Shipping: Blue Ice