Human Von Willebrand Factor (VWF) ELISA KIT (1 x 96 wells)

SKU EA102520

For quantitative detection of human VWF-A2 in cell culture supernates, serum and plasma (heparin, EDTA).

Product Data

• Species: Human
• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human VWF-A2
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 312pg/ml-20.000pg/ml
• Specificity: This kit is used for quantitative detection of Human VWF-A2
• Sensitivity: <50pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- human VWF-A2 antibody | 1
  • Lyophilized recombinant human VWF-A2 standard | 20 ng/tube×2
  • Biotinylated anti- human VWF-A2 antibody | 100 μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 100 μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12 ml
  • ABC diluent buffer | 12 ml
  • TMB color developing agent | 10 ml
  • TMB stop solution | 10 ml
  • Wash Buffer (25x) | 20 ml
  • Adhesive cover | 4
• Background: Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of plateletvessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.
• Gene Symbol: VWF
• Locus ID: 7450
• Shipping: Blue Ice