Human DR3 (TNFRSF25) ELISA KIT (1 x 96 wells)

SKU EA102414

For quantitative detection of human TNFRSF25 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Product Data

• Species: Human
• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for human TNFRSF25
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 312pg/ml-20000pg/ml
• Specificity: This kit is used for quantitative detection of human TNFRSF25
• Sensitivity: <10pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- Human TNFRSF25 antibody | 1
  • Lyophilized recombinant human TNFRSF25 standard | 20ng/tube×2
  • Biotinylated anti- human TNFRSF25 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.
• Gene Symbol: TNFRSF25
• Locus ID: 8718
• Shipping: Blue Ice