Human Growth hormone receptor (GHR) ELISA KIT (1 x 96 wells)

SKU EA102332

For quantitative detection of human GHR in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Product Data

• Species: Human
• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for human GHR
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of human GHR
• Sensitivity: <15pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- human GHR antibody | 1
  • Lyophilized recombinant human GHR standard | 10ng/tube×2
  • Biotinylated anti- human GHR antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Growth hormone receptor is a protein that in humans is encoded by the GHR gene. It is mapped to chromosome 5p13.1-p12. This gene encodes a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization (the receptor may however also exist as a pre-assembled non-functional dimer) and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature (proportional dwarfism).
• Gene Symbol: GHR
• Locus ID: 2690
• Shipping: Blue Ice