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Human soluble CD5 ELISA Kit

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SKU
EA100720
Human soluble CD5 ELISA Kit
  $1,080.00
2 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human soluble CD5
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6 pg/ml- 1000 pg/ml
Sample Type Cell culture supernates, Cell lysates, serum and plasma (heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human CD5
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-human CD5 antibody | 1
  • Lyophilized recombinant human CD5 standard | 10ng/tube x 2
  • Biotinylated anti-human CD5 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
Background CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli(such as bacterial proteins) and not by normal tissue proteins.
Gene Symbol CD5
Locus ID 921
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Chimeric antigen receptor-induced antigen loss protects CD5.CART cells from fratricide without compromising on-target cytotoxicity
Ma, Woods, Burkhardt et al
Cell Rep Med (2024) 5 (7), 101628 DOI: 10.1016/j.xcrm.2024.101628
Cited by 14
AI Summary: OriGene's Human soluble CD5 ELISA Kit (Cat#EA100720) was used in this study to measure soluble CD5 levels, contributing to the evaluation of CD5 protein expression and internalization in T cells.
Snippet: Plus Micro Kit Qiagen Cat#74034 SuperScript III First-Strand Synthesis SuperMix Invitrogen Cat#18080-400 HiScribe T7 New England Biosciences Cat#E2040S Human soluble CD5 ELISA Kit OriGene Cat#EA100720 CD3 Microbeads, human Miltenyi Biotec Cat#130-050-101 Chromium Next GEM Single Cell 5′ Kit v2 10x Genomics Cat#1000263 5′ Feature Barcode Kit 10x Genomics
FigureFigureFigure
ELISA
Evaluation of the frequency of CD5+ B cells as natural immunoglobulin M producers and circulating soluble CD5 in patients with bladder cancer
Roudafshani, Jazayeri, Mahmoudi et al
Mol. Biol. Rep. (2019)
AI Summary: OriGene’s Human soluble CD5 ELISA Kit was used to measure serum sCD5 levels in patients with bladder cancer, enabling the study to assess the diagnostic potential of soluble CD5 as an immune marker.
Snippet: SCD5 ELISA Human soluble CD5 ELISA Kit (OriGene) was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD5 has been precoated onto 96-well plates
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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