Human Chitinase 3-like 1/YKL-40 ELISA Kit

SKU EA100620

Human Chitinase 3-like 1/YKL-40 ELISA Kit

Product Data

• Species: Human
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human Chitinase 3-like 1/YKL-40
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Sample Type: Cell culture supernates, serum, plasma(heparin, EDTA) and urine.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human Chitinase 3-like 1
• Sensitivity: <10pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human Chitinase 3-like 1 antibody 1
  • Lyophilized recombinant human Chitinase 3-like 1 standard 10ng/tubex2
  • Biotinylated anti- human Chitinase 3-like 1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
• Gene Symbol: CHI3L1
• Locus ID: 1116
• Shipping: Blue Ice