Human IL-17 ELISA Kit (48-well)
| Product Data | |
| Species | Human |
|---|---|
| Format | 48-well strip plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Length | 3 hours |
| Signal | Colorimetric |
| Curve Range | 15.625-1000pg/ml |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Specificity | Natural and recombinant Human IL-17 Ligand |
| Sensitivity | 7pg/mL |
| Reactivity | Human |
| Interference | No significant interference observed with available related molecules. |
| Components |
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| Background | IL-17, originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) , is produced by activated T lymphocytes, primarily by memory T cells . IL-17 appears to mediate communication between the immune system and the hematopoietic system. IL-17 is a disulfide-linked homodimer . Each polypeptide has 155 amino acid (aa) residues (predicted mass = 17.5 kDa), including a 19 aa residue hydrophobic leader sequence . There are six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins . The aa sequence of human IL-17 is 63% and 58% identical to mouse and rat IL-17 and 72% identical to the thirteenth ORF of Herpesvirus saimiri . There is at least some species specificity for in vitro action on bone-marrow stromal cells . IL-17 mediation of T cell communication with the hematopoietic system is suggested by two observations. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-.B-mediated mechanism . IL-6 in turn promotes development of granulocyte/macrophage colonies, and G-CSF directs development of neutrophils . IL-17 also enhances proliferation of partially activated T cells and upregulates nitric oxide (NO) production in osteoarthritic cartilage . |
| Gene Symbol | IL17A |
| Locus ID | 3605 |
| Standard Curve |
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| Shipping | Blue Ice |
| Datasheet | |
| SDS | |