Carrier-free (BSA/glycerol-free) Mouse monoclonal eRFP antibody, clone OTI7C8

CAT#: CF180047

Carrier-free (BSA/glycerol-free) Mouse monoclonal eRFP antibody, clone OTI7C8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eRFP (TA180047, 1:2000).

Specifications

Product Data

• Clone Name: OTI7C8 (formerly 7C8)
• Applications: WB
• Recommended Dilution: WB: 1:2000
• Target: eRFP
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: This antibody is raised against a recombinant protein of eRFP expressed in HEK293 cells.
• Isotype: IgG2b
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: Stable for 1 year at -20°C from date of shipment.
• Predicted Protein Size: 27.0 kDa