GFP Goat Polyclonal Antibody

CAT#: AB0020-500

Goat Polyclonal Anti-GFP Antibody

Product Images

Anti-GFP Ab at 1/1,000 dilution; HEK293 transfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

• 

  Primary fibroblasts transduced with Ad GFP-Rab1a and immunostained with with GFP Ab;

• 

  AtT-20 transfected with GFP-Rab27a and immunostained with with GFP Ab;

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500-1:5,000; IF 1:50-1:1,000; IHC (paraffin) 1:50-1:1,000; IHC (frozen) 1:50-1:1,000
• Target: GFP tag
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified recombinant peptide produced in E. coli.
• Isotype: IgG
• Formulation: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Concentration: 2mg/ml
• Purification: epitope affinity purified
• Conjugation: Unconjugated
• Background: Green Fluorescent Protein
• Note: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.