Bovine IgG F(ab')2 Antibody

SKU TA396545

Bovine IgG F(ab')2 Antibody

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: WB: 1:2,000 - 1:10,000; IHC: 1:1,000 - 1:5,000; ELISA: 1:100,000
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Bovine IgG F(ab')2 fragment
• Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c).
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
• Concentration: 100 mg/mL - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Shipping: Ambient
• Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.